免疫性血小板减少症（ITP）发病主要与以下因素有关：血小板表面抗原耐受缺失、血小板破坏加速以及血小板生成受损。越来越多的研究发现，CD4+Th细胞亚群尤其是Th17细胞和Treg细胞亚群的平衡紊乱在其发病过程中起重要作用；而Notch信号通路在造血干细胞分化、巨核细胞发育成熟过程中起关键作用。基于假说，本研究以ITP模型鼠为载体，采用FCM、 Real-time Quantity PCR、Western Blot等技术检测Th17/Treg及其分化途径相关转录因子及细胞因子表达以及Notch家族受体、配体，线性分析指标间关系以及与血小板破坏/生成的关系，试图解析其在免疫性血小板减少症中的发病作用，阐释确有疗效的益气养阴清热化瘀方及其拆方是否调节Th17/Treg失衡，调控Notch信号通路，进而抑制血小板的破坏，促进血小板生成发挥免疫调节作用，以期为其治疗ITP提供科学的实验依据。

Pathogenesis of immune thrombocytopenia is mainly related to the following factors: a loss of tolerance to platelet antigens and a phenotype of accelerated platelet destruction and impaired platelet production. Increasing number of studies found that CD4+Th subset especially Th17 cells and Treg cell subsets balance disturbance play an important role in the pathogenesis of ITP; And Notch signaling pathways plays a key role in the hematopoietic stem cell differentiation, and the megakaryocyte maturation process. Based on the hypothesis, in this study the rat model of ITP as the carrier, We will use the FCM, Real-time Quantity PCR, Western Blot and other technology to detect Th17/Treg and their differentiation pathway related transcription factor and cytokine expression, and the relationship among the receptors, ligands and linear analysis of indicators of Notch family,and the relationship between the platelet destruction and production.trying to resolve its role in the onset of immune thrombocytopenia, Interpret that whether the efficacy Yiqiyangyin Qingre Huayu Recipe and its decomposed can regulate the imbalance of Th17/Treg, the Notch signal pathway, thereby inhibiting platelet destruction, promoting platelet production to play immunomodulatory effects, in order to provide the scientific experimental basis for the treatment of ITP.