川崎病(KD)以免疫系统活化和血管内皮系统广泛损害为特征，尤以冠状动脉损伤最为严重，成为儿童后天获得性心脏病的主要原因之一，然而，KD的病因及致病机制至今不明。我们前期研究发现几种miRNA联合起来可能作为KD特异性诊断标志物，从中进一步筛选，发现miR-197-3p对人冠状动脉内皮细胞（HCAEC）活性和周期有显著影响，在KD患儿血清刺激下该miRNA在HCAEC中显著上调，同时也被大量分泌。因此，我们推测该miRNA在KD冠状动脉内皮细胞损伤中发挥重要功能及调控机制。因此，在此工作基础上，本课题拟通过在KD患儿血清作用下在HCAEC中对miR-197-3p表达进行干预,筛选和确定miR-197-3p调控的靶基因，探索miR-197-3p在类似KD环境下对HCAEC功能的影响及其调控机制，阐明miR-197-3p对KD冠状动脉内皮细胞调控的分子机制，为KD致冠状动脉损伤防治提供理论依据。

Kawasaki Disease (KD) is characterized by activation of immune system and general lesion of vascular endothelial system, especially the coronary artery lesion. It has become one of the major cause of children acquired heart disease. Yet the etiology and pathogenesis of KD is unknown, our previous study found that several miRNA may jointly serve as specific diagnostic biomarker of Kawasaki disease. Among them, we further found that miR-197-3p have significant effects on the viability and cell cycle of human coronary artery endothelial cells (HCAEC). This miRNA is highly up-regulated and highly secreted in HCAEC when stimulated by KD serum. Therefore, we hypothesized that this miRNA plays an important role in lesion of the coronary artery endothelial cells of Kawasaki Disease. We aim to identify miR-197-3p target genes and clarify the regulatory mechanism by manipulating the expression of this miRNA in HCAEC in the context of KD. This will provide insights on the mechanistic effect of miR-197-3p in coronary artery endothelial cells of KD, offering theoretical basis for the prevention and treatment of KD-derived coronary artery lesion.