中医药学者长期认为 "清热解毒"与"抗病原微生物"等同，但这一"等同的机制"难以全面阐明其作用的科学内涵，我们认为清热解毒包括"清热"和"解毒"功效，清热解毒药通过解热、抗炎发挥"清热"功效，通过抗病原微生物发挥"解毒"功效。基于清热解毒药抗补体活性与防治以热证为特点的感染性疾病的高度关联性，我们提出，清热解毒药通过抑制补体的过度激活来解除感染性疾病的发热和炎症，从而发挥其"清热"功效。江苏道地药材野马追有抗炎、抗菌抗病毒作用，我们进行的抗补体活性测试表明野马追有强抗补体活性，我们认为野马追通过抗补体而清热，在此基础上，本项目将体外抗补体活性导向分离清热成分，内毒素发热模型验证其体内解热作用，ELISA法验证其抗炎作用，从而阐明野马追的"清热"功效及药效物质；体外抗菌抗病毒测试，阐明野马追的解毒药效物质。本项目将为野马追的质量控制提供科学依据，并可能为清热解毒药的清热药效物质研究提供新思路

Traditional Chinese medicine scholars have long considered that the "heat clearing and detoxicating" effect and the "anti-pathogenic microorganism" effect equal, but that "equivalent mechanism" is difficult to fully elucidate its functions from the scientific point. We think heat clearing and detoxicating including "heat clearing" effect and "detoxicating" effect. We present the heat clearing and detoxicating drugs play " heat clearing" effect by antipyretic and anti-inflammatory,detoxicating effect through anti-pathogenic microorganisms. Based on the heat clearing and detoxicating drugs of anti-complement activity and control to heat syndrome characterized by the infectious diseases of the high degree of relevance, we propose the heat clearing and detoxicating drugs play the "heat clearing" effect by inhibiting complement activation to remove infectious diseases of fever and inflammation. Authentic and superior medicinal herbal of jiangsu Eupatorium lindleyanum DC. has anti-inflammatory, antibacterial and antiviral action. We performed the anti-complementary activity test shows that Eupatorium lindleyanum DC. has strong anti-complementary activity. We think Eupatorium lindleyanum DC.play the heat clearing effect by anti-complement. Based on the above experiments we will isolate and get the fractionations and compositions of "heat clearing" guided by anti-complement activity in vitro. We will validate the antipyretic effect of the heat clearing compositions by the endotoxin fever model in vivo, at the same time we will verify the anti-inflammatory effect of the heat clearing compositions by ELISA method. Based on the above research results we can elucidate the "heat clearing" effect and its pharmacodynamic materials of Eupatorium lindleyanum DC.. In addition we can elucidate the detoxification efficacy substance of Eupatorium lindleyanum DC. by the antibacterial and antiviral test. Both from theory or experiment we can all get the "heat clearing" and "detoxicating" pharmacodynamic materials of Eupatorium lindleyanum DC. based on traditional Chinese medicine theory. The research achievement of this project will provide the scientific basis for Eupatorium lindleyanum DC. quality control and the new ideas and research methods on heat clearing effect and its pharmacodynamic materials from the heat clearing and detoxicating drugs. At the same time for the "heat clearing" effect and the "detoxicating" effect of collaborative treatment of infectious diseases, which laid the foundation of scientific research.