硼是植物必需的微量营养元素。油菜素甾醇（BR）促进生长发育，而茉莉酸（JA）则提高植物抗逆而负调控生长。我们研究发现：缺硼诱导BnaA3.NIP5;1和BnaC4.BOR1;1c基因表达，增强了硼的吸收和转运能力；同时，缺硼下油菜素内酯含量下降，BR合成和信号途径中的基因发生差异表达，其中BnBZR1基因敲除的转基因材料对缺硼胁迫更敏感；缺硼显著提高了JA含量，JA合成抑制剂缓解了缺硼的胁迫。然而，BR参与缺硼胁迫反应的机制/途径?缺硼下BR与硼吸收利用的关系？尚未有报道。由此，本研究利用油菜硼高效、低效品种和相关转基因材料，从基因表达、蛋白互作、硼吸收利用、BR和JA含量变化、根尖根系和株型生长发育等方面，研究BR参与油菜缺硼反应的节点基因和调控途径，及其与硼吸收转运基因表达和JA负调控的协同互作关系。期望获得植物硼营养与BR调控关系的原创性成果，并为油菜生产的株型调控提供潜在技术。

Boron (B) is an essential micronutrient for plants. Brassinosterol (BR) promotes plant growth and development, while jasmonic acid (JA) improves plant stress resistance and negatively regulates growth. We found that B deficiency induced the expression of BnaA3.NIP5 and BnaC4.BOR1;1c genes, which enhanced the ability of B uptake and transport. Meanwhile, brassinolide (BL) content was decreased and the genes in BR synthesis and signaling pathway showed differentially expression when B deficiency occurred, and the BnBZR1 gene knockout transgenic lines were more sensitive to B deficiency. B deficiency significantly increased JA content, and JA synthesis inhibitor alleviated B-deficient stress. However, the mechanism and pathway of BR response to B-deficient stress and its relationship with B uptake and utilization have not yet been reported. In this study, the nodal genes and regulatory pathways of BR involved in B deficiency response in Brassica napus will be studied in terms of gene expression, protein interaction, uptake and utilization of boron, BR and JA concentration changes, and the growth and development of root tip, root system and plant type. The regulation relationships between BR & B-related gene expression, and BR & JA negative regulation will also be studied. It is expected to obtain original results on the relationship between plant B nutrition and BR regulation, and provide potential technology for plant type regulation in rapeseed production.