植物激素是植物体内的微量信号分子，它控制了植物的整个生长发育过程。硼是植物生长发育所必需的微量营养元素，植物缺硼引发多个植物激素含量和分配的显著变化。然而，植物缺硼的形态和生理反应与植物激素信号调控关系的机制尚未有报道。本申请项目在已有研究的基础上，以甘蓝型油菜抗缺硼胁迫的硼高效品种和对缺硼敏感的硼低效品种、以及拟南芥硼吸收转运和植物激素合成代谢缺陷的突变体为材料，设置不同缺硼胁迫处理，以茉莉酸（JA）、生长素（IAA）和细胞分裂素（CTK）为重点，研究1）根、茎、叶、蕾、花、荚果等组织器官植物激素含量和分配的时空动态变化；2）植物激素合成、运输和降解代谢过程关键酶活性及其调控基因表达的变化；3）以及与缺硼胁迫下活性氧积累的关系。研究结果从基因和蛋白水平上揭示油菜低硼胁迫反应与植物激素调控关系的机理、植物激素在抗缺硼胁迫中的重要调控作用、以及缺硼反应、激素变化与活性氧积累三者间的因果关系。

Phytohormones are signal molecules presenting in trace quantities in plants, which mediate the whole processes of plant growth and development. Boron (B) is an essential micronutrient for plant growth and development, and B deficiency causes significant changes in concentration and distribution of phytohormones in plants. However, the mechanism on relationship between the response in morphology and physiology to B deficiency and phytohormones regulation is still unknown. In the project, Brassica napus B-efficient and inefficient cultivars, Arabidopsis mutants in B uptake and transport, phytohormones biosynthesis and metabolism will be employed in a series of low-B stress experiments based on our previous research bases. The study focusing on JA, IAA and CTK includes 1) analyzing the temporal-spatial-dynamic changes in concentration and distribution of phytohormones in root, stem, leaf, flower, bud and fruit; 2) detecting the key enzymes activity and genes expression involving in the pathway of phytohormones biosynthesis and metabolism; 3) investigating the relationship between phytohormones change and reactive oxygen species accumulation. The aim is to reveal the mechanism on relationship between the response of Brasscia napus to B deficiency and phytohormones signaling regulation, the regulation role of phytohormones in Brassica napus resisting B deficiency, and the relationship among B-deficient response, phytohormones change and reactive oxygen species accumulation.