大葱是中国传统蔬菜，蓟马是影响大葱产量和品质的主要害虫，而大葱表皮蜡质与蓟马抗性相关。因此，研究大葱表皮蜡质形成分子机制具有重要的科学意义。项目组前期，发现了一株缺乏超长链蜡质的大葱少蜡突变体gl，结合转录组和高通量KASP基因分型技术，发现CER2-LIKE1基因发生移码突变是导致gl突变体少蜡的关键原因。本研究拟利用已建立的大葱遗传转化体系，通过功能互补和基因敲除实验，验证CER2-LIKE1调控大葱蜡质代谢，特别是超长链蜡质合成的分子功能，并鉴定转基因大葱的蓟马抗性；利用免疫共沉淀和酵母双杂交技术，挖掘CER2-LIKE1调控蜡质代谢的基因网络,结合酵母异源表达技术，分析CER2-LIKE1在超长链脂肪酸合成中的生化机制。本项目的完成将阐明大葱表皮超长链蜡质合成的分子机制，为进一步搞清大葱抗蓟马机制、利用分子设计育种技术创制抗蓟马种质资源奠定理论基础。

Welsh onion (Allium fistulosum) is a traditional vegetable in China. Thrips are the main pests affecting the yield and quality of Chinese welsh onion, and its epidermal wax is related to the thrip resistance . Therefore, study on the molecular mechanism of epidermal wax formation in welsh onion is of great significance . In the early stage of the project, gl, a glossy mutant lacking very-long chain waxes, was found in welsh onion. Based on transcription and high-throughput KASP genotyping techniques, it was found that the frameshift mutation of CER2-LIKE1 gene was the key to result in lacking waxes of the gl mutant . The purpose of this study is to verify the molecular function of CER2-LIKE1 in wax metabolism regulation, especially in very long chain waxes biosynthesis, and to identify the thrip resistance of transgenic welsh onion by the established genetic transformation system of welsh onion, and functional complementarity and gene knockout experiments. In addition, the application of immunoprecipitation and yeast two-hybrid technology is to explore the gene network of CER2-LIKE1 regulating wax metabolism, and the utilization of heterogeneous expression in yeast is to analyze the biochemical mechanism of CER2-LIKE1 in very -long chain fatty acids (VLCFAs) biosynthesis. The project will clarify the molecular mechanism of very-long chain waxes synthesis in welsh onion epidermis, providing theoretical basis for further understanding the mechanism of anti-thrips and creating anti-thrips germplasm resources by molecular design breeding in welsh onion.