前列腺癌骨转移是其死亡的主要原因，而肿瘤细胞定植是骨转移过程的第一步。我们前期发现SERPING1在前列腺骨转移组织中低表达，发表的文章表明，与非癌组织相比，SERPING1在前列腺癌中表达也降低并与远处转移及预后呈负相关。预实验发现：SERPING1高表达可抑制肿瘤细胞迁移及侵袭，并在体内实验中抑制肿瘤细胞骨转移定植。进一步实验发现，SERPING1在核内结合并抑制HDAC1，通过调控组蛋白乙酰化参与调控骨转移定植。据此，我们提出假设：SERPING1通过核内结合并抑制HDAC1活性，促进RGS13组蛋白H3乙酰化，RGS13转录增加后负性调控RANKL转录，从而抑制前列腺癌骨转移定植。本课题拟采用CHIP-seq、激光共聚焦及构建体内骨转移模型等技术，证明SERPING1通过组蛋白乙酰化调控RGS13抑制前列腺癌骨转移定植的具体机制，为前列腺癌骨转移的诊治提供新的科学依据。

Bone metastasis of prostate cancer is the main cause of death, and tumor cell colonization is the first step in the process of bone metastasis. We previously found that SERPING1 was low expressed in prostate bone metastases. The published articles showed that SERPING1 expression in prostate cancer was also lower than that in non-cancer tissues, and was negatively correlated with distant metastasis and prognosis. Preliminary experiments showed that high expression of SERPING1 could inhibit the migration and invasion of tumor cells, and inhibit bone metastasis and colonization of tumor cells in vivo. Further experiments showed that SERPING1 binds in the nucleus and inhibits HDAC1, and participates in the regulation of bone metastasis and colonization by regulating histone acetylation. Accordingly, we hypothesized that SERPING1 promotes the acetylation of RGS13 histone H3 through intranuclear binding and inhibiting HDAC1 activity, and negatively regulates the transcription of RANKL after the increase of RGS13 transcription, thereby inhibiting bone metastasis and colonization of prostate cancer. In this study, CHIP-seq, laser confocal imaging and bone metastasis model in vivo were used to demonstrate the specific mechanism of SERPING1 regulating RGS13 by histone acetylation to inhibit bone metastasis and colonization of prostate cancer, and to provide a new scientific basis for the diagnosis and treatment of bone metastasis of prostate cancer.