宿主细胞的外被体蛋白Ⅰ（coatmer proteinⅠ，COPⅠ）介导高尔基体-内质网的逆向运输，参与了多种病毒的转运与复制过程。我们研究发现COPⅠ对猪瘟病毒（CSFV）的复制有显著影响，但其作用机制还不清楚。本项目拟在前期研究基础上，用real-time PCR、Western blot、免疫荧光等技术，分析CSFV结构蛋白和非结构蛋白与COPⅠ之间的表达量及胞内定位的关系，明确COPⅠ介导CSFV胞内转运的方式；通过siRNA技术和慢病毒过表达技术，检测细胞内COPⅠ蛋白表达量的变化对CSFV复制的影响，进而确定调控CSFV复制的关键蛋白；通过激光共聚焦、Co-IP、GST pull-down等技术，分析COPⅠ转运CSFV的复合体组成及互作模式，进而揭示COPⅠ调控CSFV在宿主细胞内的转运和复制的机制，为阐明CSFV建立感染的机制提供新的科学资料。

Intracellular coatmer proteinⅠ(COPⅠ) mediate the protein retrograde transport of Golgi-to-ER and are involved in viral repliction. Our previous studies showed that the COPⅠhave closely relationship with the replication of classical swine fever virus (CSFV). However, the mechanism remains unclear. Based on our previous study, this study will investigate the expression and location relationship between COPⅠand CSFV structural and non- structural proteins. By real-time PCR, Western blot and immunofluorescence, the characteristic of CSFV intracellular transport will be explored. In addition, the siRNA and lentivirus will be used to change the expression of COPⅠproteins and the key proteins which has significance effect on CSFV replication will be make certain. Furthermore, the composition of COPⅠ-CSFV transport complex and their interaction pattern will be studied by Co-IP, GST pull-down and laser scanning confocal microscope. This study could deepen our understanding of CSFV intracellular transport and replication mechanisms. In conclusion, our study will provide new scientific data for elucidating the mechanism of CSFV infection.