咖啡豹蠹蛾是一种食性杂、为害重的枝干钻蛀害虫，在武陵山区猖獗为害珍贵的药用植物杜仲。我们前期的观察发现：嗅觉在咖啡豹蠹蛾对寄主植物的识别过程中发挥了关键作用，但是该虫将杜仲作为寄主植物的嗅觉识别机制仍不清楚。本项目拟从咖啡豹蠹蛾中鉴定得到两类特异性嗅觉蛋白基因（气味结合蛋白OBPs和化学感受蛋白CSPs），分析它们在咖啡豹蠹蛾寄主选择关键时期的表达规律；然后通过分子模拟筛选参与杜仲气味化合物识别的咖啡豹蠹蛾OBPs/CSPs，并利用荧光竞争结合实验测定这些嗅觉蛋白与杜仲气味化合物的结合特性。从而揭示咖啡豹蠹蛾将杜仲作为寄主植物的嗅觉识别机制，以期为开发基于嗅觉功能的咖啡豹蠹蛾无公害治理手段提供科学数据。

Zeuzera coffeae is a notorious wood-boring pest of a wide rang of ligneous plants and seriously infesteds Eucommia ulmoides, a valuable medicinal plants in Wuling mountain. Our previous studies found that the olfactory system of Z. coffeae played a key role in host plant selection. However, the olfactory mechanism of Z. coffeae choosing E. ulmoides as host plant is still unknown. In this project, two kinds of specific olfactory proteins, odorant binding proteins (OBPs) and chemosensory proteins (CSPs), will be identified from Z. coffeae and their expression profiles during host plant selection will be determined. Molecular docking will be applied to screen E. ulmoides odorants binding OBPs and CSPs, and their binding properties will be determined by fluorescence binding assays. Our work will reveal the olfactory selection of Z. coffeae to E. ulmoides and provide information for developing environmental friendly pest management strategy base on olfactory system of Z. coffeae.