慢性移植物抗宿主病的发病率正逐年上升，已成为异基因造血干细胞移植后患者非移植相关死亡的主要原因，并已成为异基因造血干细胞移植最难处理的并发症。炎性因子失衡和纤维化在慢性移植物抗宿主病的发病机制中占据了主导性作用。研究表明分子氢具有抗炎、抗纤维化、抗氧化作用，因此我们推测分子氢对慢性移植物抗宿主病可能具有治疗作用。近年，我们在国际上首先报道了分子氢对急性移植物抗宿主病的治疗作用，并开展了分子氢治疗慢性移植物抗宿主病的初步实验。本项目将在前期实验的基础上，系统研究分子氢对小鼠慢性移植物抗宿主病的治疗作用，包括对小鼠生存率、皮肤、肠道、肝脏病变的影响；另将从细胞因子、淋巴细胞亚群、DNA分子损伤与修复、细胞周期、细胞凋亡、氧化损伤、细胞信号通路、细胞活化等多个层面，系统探讨其作用机制。本项目将为慢性移植物抗宿主病的治疗提供新思路，对造血干细胞移植及血液病学研究具有重要的理论价值及潜在的应用前景。

The incidence of chronic graft-versus-host-disease is rising year by year, which has become the leading cause of non-transplantation related death and has become the most difficult complication of allogeneic hematopoietic stem cell transplantation to deal with. Inflammation and fibrosis play dominant roles in the pathogenesis of chronic graft-versus-host-disease. Studies have shown that molecular hydrogen has anti-inflammatory, antioxidant, anti-fibrosis effects. Therefore, we hypothesized that molecular hydrogen may have therapeutic effects on chronic graft-versus-host-disease. Recently, we firstly reported the therapeutic effects of hydrogen on acute graft-versus- host disease and carried out preliminary experiments on the therapy effects of hydrogen on chronic graft-versus-host disease. Based on the experimental results previously, we will study the therapeutic effects of hydrogen on chronic graft-versus- host disease, including the survival rate of mice with chronic graft-versus-host disease and its therapeutic effects on skin, intestine, liver lesions. We will also study the mechanism of its therapeutic effects by multiple experiments on cytokines, lymphocyte subsets, DNA damage and repair, cell cycle, cell apoptosis, oxidative damage, cell signaling pathways, cell activation, et al. This project will provide new ideas on the treatment of chronic graft-versus-host disease and has important theoretical values and potential application prospects on the study of hematopoietic stem cell transplantation and hematology.