石墨烯多孔支架附载生长因子重建兔下颌骨缺损及其机制研究

颌骨缺损严重影响美观和生理功能，制备适合的支架材料构建组织工程骨进行修复是当前紧迫的需求。石墨烯具有卓越的机械性能、超高的比表面积和吸附能力，我们已发表的研究还首次证明其骨诱导性和降解性，揭示了石墨烯巨大的骨再生潜力。但是石墨烯用于骨缺损修复重建的作用及具体成骨机制尚不清楚。本课题将通过三项研究从体内外深入了解石墨烯能否成为有效地附载和缓释成骨生长因子的组织工程支架材料来促进骨再生：1）构建石墨烯多孔支架并评价其理化特性及促进成骨能力；2）研究石墨烯体外吸附和缓释成骨生长因子的能力及促进成骨的作用；3）通过与附载外源性成骨生长因子的石墨烯多孔支架对比，研究石墨烯是否也能吸附并浓集内源性成骨生长因子来修复重建兔下颌骨缺损。本课题将为进一步开发石墨烯纳米材料成为活性的内源性成骨生长因子载体用于重建骨缺损的临床研究提供理论基础，从而为颌骨缺损修复提供新一代安全而有效的生物支架材料。

Jaw defect seriously affects the appearance and physiological function of human being. It is urgent to find a suitable biological scaffold to repair the defect. We known that graphene has excellent mechanical properties, superhigh surface area and adsorption capacity. Our published article has verified for the first time that graphene has osteoindcutivity and biodegradability, indicating its great potential for bone regeneration. But the bone regeneration function and specific osteogenic mechanisms of graphene are not clear. This topic will be researched deeply to understand how graphene enhaces bone regeneration by three studies: 1) to establish graphene porous scaffold and evaluate its physicochemical properties and osteogenic ability; 2) to study adsorption and sustained release of osteogenic growth factors of graphene; 3) to study whether graphene can adsorb and concentrate endogenous osteogenic growth factors to promote bone repair by comparing with the bone regeneration abilityof graphene porous scaffold carring exogenous osteogenic growth factors. This project will provide theoretical basis for further clinical studies on the reconstruction of bone defect by using graphene nano-material as an active endogenous osteogenic growth factors carrier, and offer a new generation safe and effective biological scaffold for jaw regeneration.