导电性石墨烯弹性支架促进功能性骨骼肌再生及其机制研究

颅颌面部骨骼肌缺损常见，使用生物活性支架来再生，是解决这一临床难题的有效途径。已知石墨烯具有导电性、回弹性、表面活性等成骨骼肌相关特性。我们预初实验构建了导电性石墨烯弹性支架，具有成骨骼肌分化性能。然而如何实现血管化、神经化的功能性骨骼肌再生？基于石墨烯表面可修饰性，提出假说：经羧基、环氧基等功能基团修饰的石墨烯支架，可以通过提高静电和范德华力促进成血管和神经生长因子的吸附，赋予支架动态长效的诱导骨骼肌内多种细胞生成的生物活性，实现骨骼肌的功能重建。拟开展：1)修饰支架材料，检测其理化性能；2)研究其生物相容性、降解性、成骨骼肌分化和收缩活性；3)评价其再生动物功能性骨骼肌能力；4)揭示其缓释多种生长因子促进骨骼肌再生的机理。从材料性能-成骨骼肌活性-功能性骨骼肌再生-成骨骼肌机制四层次深入探索，以期利用石墨烯促进功能性骨骼肌再生并阐明其机理，为重建功能性骨骼肌的临床应用研究提供理论基础。

The defect of craniomaxillofacial skeletal muscle is a common clinical disease. It is urgent to find bioactive scaffolds to regenerate skeletal muscle. It is known that graphene has many properties related to regeneration of skeletal muscle, such as electrical conductivity, resilience, surface bioactivity, etc., suggesting its potential for skeletal muscle regeneration. In our preliminary experiment, we have prepared a conductive graphene elastic scaffold that can induce stem cells to differentiate into skeletal muscle. However, how to achieve vascularized and neurogenic functional skeletal muscle regeneration? Based on the surface modifiability of graphene, the hypothesis is put forward that graphene scaffolds modified by carboxyl, epoxy and other functional groups can promote the adsorption of vascular and nerve growth factors by increasing static electricity and van der Waals force. We are going to further endow the scaffold material with dynamic and long-term biological activity and realize the functional reconstruction of skeletal muscle. This topic will be researched deeply to understand how graphene combined with vascular and nerve related growth factors enhances functional skeletal muscle regeneration by four studies: 1) To modify scaffold material and evaluate its physicochemical properties; 2) To study the compatibility, degradability, differentiation and contraction ability of skeletal muscle of the scaffold; 3) To evaluate the scaffold material as an artificial substitute to repair skeletal muscle defect of animal; 4) To reveal the mechanism of how the scaffold slowly releases growth factors to promote functional skeletal muscle regeneration. This project based on four aspects will show that graphene can promote the regeneration of functional skeletal muscle and clarify its mechanism, providing a theoretical basis for the clinical application of functional skeletal muscle reconstruction.