飞秒激光小切口透镜取出术(SMILE)已成为角膜屈光手术的主流术式。角膜扩张是屈光手术严重威胁视力的并发症，主要与角膜变薄后生物力学稳定性减弱有关。课题组前期利用胶原交联术增加了角膜力学强度，可用于联合SMILE预防术后角膜扩张。目前SMILE联合胶原交联术的生物力学效应及创伤愈合机制仍有待阐述。为此，本研究将对兔眼行SMILE术，分别保留安全、临界、过薄厚度的残余基质床，进行不同能量参数的快速角膜胶原交联。主要研究内容包括：① Corvis ST、ORA、角膜单轴拉伸实验、层间粘合力试验检测角膜生物力学效应；② 活体共聚焦显微镜观察基质细胞、神经修复规律，透射电镜观察角膜超微结构变化，TUNEL检测细胞凋亡，PCR、Western blot、免疫组化检测角膜愈合相关基因、蛋白表达。最终明确手术关键参数及其效应的量-效规律，为薄角膜及高度近视患者行SMILE联合胶原交联手术提供理论依据。

Femtosecond laser small incision lenticule extraction (SMILE) has become the mainstream of corneal refractive surgery. Corneal dilatation is one of the complications of refractive surgery which serious threat visual acuity, mainly with the corneal thinning and weaken of biomechanical stability. The use of collagen cross-linking to improve the mechanical stability of the cornea, can be used for combined with SMILE surgery to improve postoperative corneal mechanical stability. The biomechanical effect and wound healing of SMILE combined with collagen cross - linking are to be discussed. In this study,rabbit eyes underwent SMILE were kept safe, critical, too thin thickness of the residual matrix bed, respectively, different energy parameters of the UV - riboflavin corneal collagen cross - linking. The main contents were as follows: (1) Corvis ST, ORA, uniaxial stretch test, intermolecular adhesion test to measure corneal biomechanical parameters; ② Confocal microscopy observation of stromal cells, nerve repair, transmission electron microscopy observation of corneal ultrastructural changes, TUNEL detection of apoptosis, PCR, Western blot, immunohistochemical detection of corneal healing related genes, protein. And finally clear the key parameters and the effect of the dose-effect law of femtosecond combine cross-linked surgery for the treatment of thin cornea and high myopia patients and provide a theoretical basis.