自噬在乙肝病毒诱导肝癌中的作用及可能的机理研究

HBV感染是肝癌发生的重要诱因之一。已有研究发现HBV感染也可以上调细胞自噬的水平，但是HBV诱导激活的自噬对细胞本身的影响尚不明确，这种自噬水平的变化可以促进HBV的复制。同时其他的研究发现，在无HBV感染的情况下，自噬是抑制肝细胞肝癌发生的一种保护性机制。但对于HBV感染的肝癌来说，HBV所上调的自噬对肝细胞肝癌的发生有何影响尚不明确。在我们的前期试验中，已经证实自噬与肝病毒复制之间有着密切的关系，并发现HBV感染的HCC患者组织中自噬水平增加。本研究通过进一步比较HBV感染和非感染的HCC患者癌和癌旁组织标本中自噬水平的变化，并与感染者血清中HBV复制水平相对应，明确HBV感染的HCC患者肿瘤样本中自噬水平的变化。同时在细胞水平进一步探讨HBV感染诱导激活的自噬现象在HCC发生中可能的机制，并将上述体外实验中的发现在肿瘤患者样本中进行重复验证。

Hepatitis B Virus (HBV) infection is one of the most important causes for hepatocellular carcinoma (HCC). Research has found that HBV infection can raise the level of autophagy both in vivo and in vitro, although the influence of activated autophagy induced by HBV is not clear, but this kind of autophagy level changes can promote HBV replication. Other research has also found that autophagy is a protective mechanism to prevent hepatocellular carcinoma without the background of HBV infection. But it hasn't been studied whether the activated autophagy induced by HBV infection is still a protective mechanism or one of its carcinogenicity. In our previous studies, we have confirmed the relationship between autophagy and HBV replication. And we also found the autophagy level is raised in HCC patients with HBV infection. In this study, we will try to further compare the autophagy level in the carcinoma tissues and the para- carcinoma tissues from the HCC patients with or without HBV infection, the different serum HBV copies will also been taken into consideration to make sure the change of autophagy in HCC patients with HBV infection. At the same time we will further explore the influence of activated autophagy induced by HBV in HCC cells in vitro, and validate in the carcinoma tissues from the HCC patients.