异黄酮具有较高的生理功能和应用价值，在大豆中含量最高，研究表明MYB转录因子参与异黄酮的合成，探明其作用机制有助于人为调控异黄酮的生物合成，但目前为止在该方向还有诸多待解决的问题。本课题组前期从大豆中筛选到6个与异黄酮合成相关的MYB转录因子，并利用酵母双杂交筛选到其中一个基因MYB12B2的互做蛋白暂命名为Pmyb12b2-1。本项目将进一步探索MYB转录因子与互作蛋白作用位点，互作蛋白对MYB转录因子的作用方式，包括对转录因子的活性，亚细胞定位及与其他靶标分子结合的影响等，进而探明Pmyb12b2-1与MYB12B2的作用机理；研究Pmyb12b2-1是否能调控其他5个与异黄酮合成相关的MYB转录因子进而调控异黄酮的生物合成；研究互作蛋白Pmyb12b2-1是否具有异形体特异性，如有，则阐释其对MYB转录因子的作用机理及作用特性。该项目的完成，对大豆异黄酮的遗传改良具有理论和实践意义。

Isoflavonoids are one of the specific forms of secondary metabolite products from leguminous plant, which has important physiological functions and applications. Soybean has the highest concentration of isoflavonoids in all legumes. The study of isoflavone biosynthesis regulation is an important topic for improving soybean quality. MYB transcription factors are involved in biosynthetic pathway understanding action mechanism could help us to regulate isoflavones synthesis. However, only little work was done in this area. .We found 6 MYB transcription factors, which had relationship with isoflavonoid biosynthesis in early work. An interacting protein Pmyb12b2-1 was obtained using yeast two-hybrid system responsible for MYB12B2. We are going to investigate interacting mechanism between MYB12B2 and Pmyb12b2-1 including binding site、activity、subcellular localization and how to affect transcription factor MYB12B2. We will carry out experiments to analyze whether Pmyb12b2-1 could work with the other 5 MYB transcription factors and how to regulate the synthesis of isoflavonoids. Experiments will be performed to detect specificity of Pmyb12b2-1 isomer and how to interact with transcription factors. Identification of MYB transcription factors and reaction protein may lead to understanding of isoflavonoid biosynthesis. Therefore, this project has practical implication contributing to genetic improvement in soybean.