棉花细胞质雄性不育恢复基因RfB精细定位与克隆

细胞质雄性不育对棉花杂种优势的利用具有重要意义，但恢复基因的育性恢复机理尚不清楚。课题组在对海岛棉恢复系细胞质雄性不育恢复基因的遗传分析发现，该恢复系属孢子体遗传，有一个显性主基因RfB控制，因此，对恢复基因RfB进行精细定位和分离，有利于阐明恢复基因RfB的遗传机理，为细胞质雄性不育在海陆杂种生产上的利用提供理论依据。本研究拟利用图位克隆的技术手段，构建海岛棉恢复系BAC库，开发新的分子标记，在含5700植株的BC1分离群体中对棉花RfB基因进行精细定位；然后利用生物信息学结合序列分析的方法在候选基因区域内预测RfB候选基因；分离得到RfB基因并对它的表达特性和育性恢复机制进行研究。

In order to clarify inheritance mechanism of fertility restoration for cytoplasmic male sterility (CMS) in a new Gossypium barbadense restorer line ‘Hai R’which is of value in the application of cotton interspecific hybrid breeding. Constucting cytoplasmic male sterile(CMS) restorer line‘Hai R’BAC library. Four BC1 segregating populations of 5700 plants,derived from cytoplasmic male sterile(CMS) restorer line ‘Hai R’were employed to analyze the inheritance and map the fertility restorer gene RfB for gene cloning.