我们在毒物分析的生物样品中发现了1-甲基海因 (1-methylhydantoin ，1-MID) 并能够在离体状态下通过肌酐生成1-MID。本研究拟通过肾衰动物模型和溺死动物模型，在模拟肌酐蓄积和厌氧菌脱氨酶催化两个条件下，研究1-MID生前死后的生物合成机制和转化动力学。用中国林蛙作为研究对象和蛤蚧作为对照物，调查动物类中药材中1-MID的生成规律，为具有中国特色的动物类名贵中药材的国家标准修订提供科学依据。同时，本研究尝试用1-MID作为溺死死因的生物学标志物和评价法医毒物分析生物检材的新鲜度，研究若干种不同化学转化类型的典型药物与检材新鲜度的正负相关性。本研究对1-MID的生物合成、动物类中药材质量控制、溺死死因判定以及解决法医毒物相关的疑难案件具有重要意义。

We found 1-methylhydantoin(1-MID) in the biological samples during the practice of forensic toxicological screening, and also 1- MID has been able to be generated from creatinine in vitro in our lab.. In this study, the biosynthesis mechanism and the transformation dynamics of 1-MID will be explored, with two animal models of both the renal failure and the drowning，to meet the necessary conditions, high creatinine level and catalysis of anaerobic bacteria. The site and pattern of 1-MID biological synthesis are researched to provide science evidence to establish the Chinese medicine national standard， with some zoogenic Chinese medicine, Rana tem poraria chensinensis David and Gekko gecko Linnaeus. In the meantime, this program devote that 1-MID is used for the biomarker to the death from drowning，and the freshness to biological samples in the legal medical poison analysis，to devote the correlation between the freshness and the typical drugs including several chemical inversion pattern. This research has important signifecance to formulate the national standard of zoogenic Chinese medicine and for forensic practice.