NB-LRR蛋白是植物中数量最多的一类抗病(R)蛋白，在多种病害的抗性中发挥重要作用。R蛋白激活后可介导一系列抗性反应，包括超敏反应(HR)的诱发，但R蛋白激活后导致HR的分子机理尚未得到彻底解析。玉米Rp1-D21编码一个CC-NB-LRR类抗病蛋白，其突变可导致组成型的HR表型，是深入解析HR诱发分子机理的理想材料。我们前期发现，内体分选转运复合体I(ESCRT-I)的关键组分UEV和VPS37与Rp1-D21介导的HR相关联，它们可与Rp1-D21的CC信号功能域结合，从而影响其介导的HR表型。在此基础上，本研究拟利用分子和生化技术系统研究UEV和VPS37调控Rp1-D21的分选与机制，并通过基因编辑与过表达技术研究它们在玉米抗病中的作用。研究结果可深入揭示植物ESCRT-I复合体对R蛋白行使功能以及调控HR的分子机理，同时可能为玉米及其它作物抗病性的遗传改良提供新策略和新基因源。

NB-LRR (Nucleotide binding-leucine rich repeat) protein is the largest number of resistant (R) proteins in plant, which plays important roles in resistance to multiple diseases. After R protein is activated, plant produces a number of defense responses, including the induction of the hypersensitive defense response (HR), however, the molecular mechanism has not been fully understood. In maize, Rp1-D21 encodes a CC-NB-LRR protein, the mutation in which shows autoacitve HR phenotype. Thus Rp1-D21 mutant is an ideal material to investigate the molecular mechanism of HR induction. We previously found that UEV and VPS37, the two key compounds in endosomal sorting complex required for transport-I (ESCRT-I), are related with Rp1-D21-mediated HR phenotype. UEV and VPS37 can bind the CC (Coiled-coil) signaling domain of Rp1-D21 to affect the HR phenotype. Based on these results, we will systematically investigate the molecular and biochemical mechanisms of how UEV and VPS37 regulate the protein sorting of Rp1-D21 and affect the HR phenotype. We will further construct the gene editing and over-expression lines for UEV and VPS37 in maize and evaluate their resistance response to the major maize diseases. This study will reveal the molecular mechanism of ESCRT-I in HR regulation and how it functions in R protein mediated disease resistance. It will provide new strategies and novel gene resources for the genetic improvement in disease resistance in maize and other crops.