通过24公顷森林样地高强度系统取样和高通量测序技术，首次系统地分析菌根真菌、病原真菌和真菌分解者在古田山亚热带森林生态系统中的多尺度分布格局及其影响因素；并将地上植物群落-真菌类群下行过程（top-down effects）与真菌类群-植物共存的上行过程(bottom-up effects)进行有机整合，评估真菌多样性对植物幼苗更新和生长的影响。其中，遍及24公顷生物多样性监测样地的多位点真菌多样性检测，用于揭示真菌多样性的格局、分布特征，并探讨植物群落和环境因素对真菌多样性的影响；同时结合森林监测样地幼苗动态数据分析真菌多样性对植物群落幼苗更新的反馈调节。本项目的结果将有助于理解亚热带森林中植物共存和多样性维持的控制机制，以及真菌多样性在其中起到的作用，为亚热带森林的生物多样性保护提供理论依据。

We aim to investigate the diversity patten of fungi surviving in litters, roots and soils in Gutianshan 24 ha plot of Zhejiang province, and their impacts on the survival and growth of plant seedling. It is the first time to simutineously elucidate the contributions of diversity of pathogen, mycorrhizal fungi and fungal decomposers to plant community co-existence. We would conduct a high-indensity sampling compaign and use a powerful molecular method (i.e.power sequencing) to estimate fungal diversity and their role in regulating plant survival, growth and co-existence. About 829 soil samples,535 root and 535 litter samples will be collected to measure fungal diversity, and also plant seedling survival and growth rate will be determined regularly per year. We would combine the data of fungal diversity with seedling dynamics in order to test contributions of fungal diversity to plant species co-existence and diversity maintenance.Overall,the project will provide insight into microbial mechanisms regulating plant species coexistence and maintenance of diversity in subtrophical forest of Gutianshan, Zhejiang province.