Diversity of root-associated fungi of Vaccinium mandarinorum along a human disturbance gradient in subtropical forests, China
Diversity of root-associated fungi of Vaccinium mandarinorum along a human disturbance gradient in subtropical forests, China
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中国亚热带森林人类干扰梯度下越橘根部相关真菌的多样性
DOI：
10.1093/jpe/rtw022
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发表时间：
2017-02
影响因子：
2.7
通讯作者：
Liang Yu
中科院分区：
文献类型：
--
作者：
Zhang Yanhua;Ni Jian;Tang Fangping;Jiang Lifen;Guo Tianrong;Pei Kequan;Sun Lifu;Liang Yu
目的埃里卡属植物的根部可以寄生多种真菌，包括埃里卡类菌根真菌（ERMF）、内生真菌、病原菌和一些功能未知的真菌。然而，真菌的这种多样性是如何对不同的人类干扰作出反应的，人们还没有很好地了解。本研究研究了不同人为干扰对桔黄色毛根真菌多样性的影响。从古天山国家级自然保护区（GNNR）的亚热带森林中提取了柑橘（V. mandarinorum）发根真菌DNA，并采用高通量测序技术，沿人为干扰梯度检测了根系相关真菌的多样性。干扰程度不同的4种森林类型分别是：原生林（OGF）、次生林（SEC I）、次生林（SEC II）和杉木人工林（PLF）。结果表明：(1)4种不同类型林分中，在毛根中检测到不同种类的真菌操作单元（OTUs），真菌门类包括子囊菌、担子菌、壶菌、肾小球菌和联合菌；（ii）林分与其他3种林分的根伴生真菌群落组成不同，2种林分的根伴生真菌群落组成相似；（iii）不同真菌科对人为干扰的响应不同：对OGF有显著偏好的真菌科是外生菌根真菌或腐生真菌，而在PLF中相对丰度较高的真菌科是植物病原真菌或腐生真菌；（iv）植物群落第一主成分（PC1）对根相关真菌群落组成有显著影响，而土壤参数对根相关真菌群落组成无显著影响。我们的研究结果有助于更好地了解白垩系植物及其真菌伴侣对人类干扰和森林管理的反应。
Aims Ericaceous plant species can host diverse fungi in their roots, including ericoid mycorrhizal fungi (ERMF), endophytes, pathogens and some species with unknown functions. However, how this diversity of fungi responds to different human disturbances is not well understood. In this study, we examined the effects of different human disturbance on fungal diversity in hair roots of Vaccinium mandarinorum , an ericaceous plant. Fungal DNA was extracted from hair roots of V. mandarinorum and high-throughput sequencing was applied to detect the diversity of root-associated fungi along a human disturbance gradient in subtropical forests in Gutianshan National Nature Reserve (GNNR) in East China. The four forest types with different disturbance regime were: old growth forest (OGF), secondary forest with once cut (SEC I), secondary forest with twice cut (SEC II) and Cunninghamia lanceolata plantation (PLF). The results showed that: (i) diverse fungal operational units (OTUs) were detected in hair roots of V. mandarinorum in the four types of forests, covering fungal phyla of Ascomycota, Basidiomycota, Chytridiomycota, Glomeromycota and Zygomycota; (ii) Community composition of root-associated fungi of V. mandarinorum in PLF was distinct from those in the other three forest types, and two types of secondary forests had similar fungal community composition; (iii) Different fungal families respond differently to human disturbances: fungal families with significant preference to OGF were ectomycorrhizal or saprophytic fungi while fungal families with higher relative abundance in PLF were plant pathogenic or saprophytic fungi; (iv) The first principal component (PC1) of plant community had a significant effect on composition of root-associated fungal community, while edaphic parameters showed no significant effect on fungal community composition in roots of V. mandarinorum . Our results help to better understand the responses of both ericaceous plants and their fungal partners to human disturbances and forest managements.
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影响因子：
3.6
作者：
David J.W. Moriarty;A. Hayward
通讯作者：
David J.W. Moriarty;A. Hayward
通过放大标记的高通量测序进行真菌社区分析 - 用户指南。
DOI：
10.1111/nph.12243
发表时间：
2013-07
期刊：
The New phytologist
影响因子：
--
作者：
Lindahl BD;Nilsson RH;Tedersoo L;Abarenkov K;Carlsen T;Kjøller R;Kõljalg U;Pennanen T;Rosendahl S;Stenlid J;Kauserud H
通讯作者：
Kauserud H
影响因子：
1.2
作者：
M. Vohník;J. Albrechtová
通讯作者：
M. Vohník;J. Albrechtová
DOI：
10.1111/j.1469-8137.2011.03703.x
发表时间：
2011-07
期刊：
The New phytologist
影响因子：
--
作者：
J. Walker;Laura Aldrich‐Wolfe;Amanda K. Riffel;Holly Barbare;Nicholas B. Simpson;Justin Trowbridge;
通讯作者：
J. Walker;Laura Aldrich‐Wolfe;Amanda K. Riffel;Holly Barbare;Nicholas B. Simpson;Justin Trowbridge;
影响因子：
2.6
作者：
Wurzburger, Nina;Higgins, Brian P.;Hendrick, Ronald L.
通讯作者：
Hendrick, Ronald L.