类风湿性关节炎(RA)是造成我国人群丧失劳动力和致残的主要疾病之一。目前已证实NF-κB信号传导通路的激活对RA病情的发展起关键作用。抑制NF-κB信号通路可有效抑制促炎因子的基因表达，诱导滑膜细胞凋亡，缓解免疫炎症反应。A20主导的NF-κB信号通路负反馈调控途径在抑制NF-κB信号通路方面起重要作用。本项目以RA动物模型(AA大鼠)为受试对象，采用电针治疗，运用光镜、放免、免疫组化、RT-PCR、Western-blot等检测手段，通过对滑膜组织炎性细胞因子；滑膜细胞胞浆内A20、RIP1、c-IAP1、c-IAP2，IκB磷酸化；核内NF-κB、A20mRNA、细胞因子等mRNA的检测，研究电针是否通过启动A20主导的NF-κB信号通路负反馈调控途径抑制NF-κB信号通路的活化，探讨电针治疗RA的抗炎机制。

Rheumatoid arthritis ( RA ) is one of the main diseases that result in loss of China's labor and disability, the prevention and control of this disease has very important significance. It has been confirmed that the activation of NF-κ B signal transduction pathway plays a crucial role in the development of RA. Inhibition of NF- κB signal transduction pathway can effectively inhibit proinflammatory cytokine gene expression, can induce apoptosis of synovial cells, can relieve the inflammatory immune response. NF-κB signal negative feedback regulation pathway mediated by A20 plays an important role in the inhibition of NF-κ B signal pathway. This project uses the animal model of RA ( AA rats ) as subjects, using electroacupuncture treatment, using morphological detection, radioimmunoassay, immunohistochemistry, RT-PCR, Western-blot and other technical means of detection, Through the the detection of synovial tissue inflammatory cytokine, A20、RIP1、c-IAP1、c-IAP2、IκB in synovial cell cytoplasm, NF-κB、A20mRNA in synovial cell core and cell factor mRNA, to study whether electro-acupuncture therapy inhibit NF- κB signal transduction pathway by activating the NF-κB signal negative feedback regulation pathway mediated by A20. So to explore the immune regulation mechanism of electroacupuncture treatment on RA.