电针调控外泌体转运miRNAs介导HIF-1α/VEGF信号系统抑制类风湿关节炎血管新生的效应机制研究

类风湿性关节炎(RA)是造成我国人群丧失劳动力和致残的主要疾病之一。血管新生在RA慢性炎症的发生和持续中起关键作用，抑制血管新生被认为是治疗RA的一种新的重要思路。“氧感知调控途径”HIF-1α/VEGF信号系统在血管新生过程中扮演重要角色，外泌体转运miRNAs介导HIF-1α/VEGF信号通路关键蛋白的转录与表达，在低氧诱导的血管重塑中发挥重要的调控作用。本项目以此为切入点，以AA大鼠为受试对象，采用电针治疗，利用转录组学、基因芯片、RT-PCR、Western-blot等多种技术手段。通过观察滑膜血管新生情况、筛查血清外泌体中与血管新生有关的差异性表达的miRNAs、检测滑膜细胞HIF-1α/VEGF信号途径关键蛋白的转录与表达、观察ECs增殖、迁移和成管情况，揭示电针是否通过调控外泌体转运miRNAs介导HIF-1α/VEGF信号系统抑制类风湿关节炎滑膜血管新生的效应机制。

Rheumatoid arthritis (RA) is one of the main diseases that cause the loss of labor and disability in China.Angiogenesis plays a key role in the occurrence and persistence of chronic inflammation of RA. Inhibition of angiogenesis is considered to be a new important way to treat RA.HIF-1α/ VEGF signal pathway, as oxygen sensing and regulating avenue, plays an important role in angiogenesis. Exosomes transport miRNAs mediate the transcription and expression of key proteins in HIF-1α/ VEGF signal pathway , which plays an important role in the regulation of hypoxia induced vascular remodeling.The project takes adjuvant arthritis rats as subjects,uses electroacupuncture treatment,uses transcriptome, gene chip, RT-PCR, Western blot and other detection .Through the observation of synovial angiogenesis, the screening of differential expression of exosomes miRNAs related to angiogenesis in serum, the detection of transcription and expression of key proteins of HIF-1α /VEGF signal pathway in synovial cells, and the observation of proliferation, migration and tube formation of ECs, to explore whether electroacupuncture can inhibit synovial angiogenesis of RA by regulating the HIF-1α /VEGF signal pathway mediated by exosomes transport miRNAs.