软骨修复中BMSC表面包被I型胶原对其“归巢”和“聚合”的影响及其机制研究

骨髓间充质干细胞（BMSC）的归巢修复作为目前最有前景的非手术软骨修复方法之一，能够早期对开放性关节损伤后表层软骨的损伤进行修复，从而预防创伤后关节炎的发生。然而，目前其仍然存在归巢率低下与定向分化障碍两大难题，限制了其修复效果。研究表明，BMSC在归巢过程中细胞团的形成与聚合障碍是目前导致上述问题的关键所在。而我们发现归巢修复中I型胶原蛋白（Col I）的缺失极有可能是导致其细胞团的形成与聚合障碍的重要原因。因此，本课题设想将Col I包被于BMSC表面，旨在证实上述理论，并为BMSC归巢修复中归巢率的提升与定向分化难题提供一种全新、有效的解决方案。

The homing regeneration of bone marrow mesenchymal stem cell (BMSC) is the most promising non-surgical cartilage regeneration approach presently. It could repair the damage of superficial cartilage after the open joint injury (OJI) at early stage and prevent the happening of the post traumatic arthritis (PTA). However, the low homing rate and the difficulty of directional differentiation has hindered its performance presently. Accroding to recent researches, it is the the limitation of formation and condensation of BMSC cell mass that result in these two problems. We found that the absence of collagen I (Col I) is highly probably the key factor which limited cell mass formation and condensation. Hence, we conceived coating the Col I on the surface of BMSC, to demonstrate this hypothesis and provide a novel, effective approach for the improvement of homing rate and directional differentiation.