程序性核糖体移码(PRF)是一种蛋白质翻译调节机制，参与调节病毒复制酶和结构蛋白的产生。研究发现，病毒的PRF效率改变，会引起病毒数量的变化，其作为抗病毒药物靶标研发已展示良好的前景。基于申请人发表在《Virology》上以及近期的研究工作得知，真菌病毒UvNV-1复制酶的翻译过程涉及程序性+1核糖体移码(+1PRF)，但其作用机制尚不清楚。本项目拟采用遗传突变分析等方法，分析UvNV-1的+1PRF信号元件（移码序列和“茎环”结构）的结构以及它们在+1PRF中的作用。另一方面，分析美洲商陆抗病毒蛋白、亚精胺和肽酰转移酶抑制剂能否通过影响UvNV-1的+1PRF效率而改变UvNV-1的复制能力。本项目的研究不仅有助于阐明真菌病毒UvNV-1的+1PRF信号元件结构和作用机制，初步评估UvNV-1的+1PRF作为抗病毒药物靶标的潜力，而且能为其他病毒中的+1PRF相关机制的研究提供重要依据。

Programmed ribosomal frameshifting（PRF）is used by many virus to regulate the production of structural and enzymatic proteins. Altering the frameshifting efficiencies disrupts the virus life cycle and eliminates or reduces virus production. Ribosomal frameshifting therefore provides a unique target on which antiviral agents can function.Based on our previous studies, Ustilaginoidea virens nonsegmented virus 1(UvNV-1) was found to be expressed via a programmed +1 ribisomal frameshift within the sequence CCC-UUU-CGA. Related sites are identified.in other virus genes that have previously been proposed to be expressed via +1 frameshifting. As these viruses infect insects (chronic bee paralysis virus), plants (fijiviruses and amalgamaviruses) and vertebrates (influenza A virus), such motifs may for a new class of +1 frameshift-inducing sequences that are active in diverse virus.Here, we applied mass spectrometry, genetic mutation analysis and other methods,to analyze element of frameshifting and the frameshifting efficiency; to investigate the function of frameshift site(CCC-UUU-CGA) and.“stem-loop”structure. Moreover, this study was to investigate the antiviral activity by altering the frameshifting efficiency of PAP , spermidine and two peptidyl-transferase inhibitors against UvNV-1. Collectively,results of this study tell us the function of frameshift site (CCC-UUU-CGA) and“stem-loop” structure and to evaluated whether programmed +1 ribosomal frameshifting of UvNV-1 as a target for antiviral agents , have laid a solid foundation for the related programmed +1 ribosomal frameshifting of virus as a target for antiviral agents.