水稻CNGCb和CNGCc调控高温胁迫反应的分子机制研究

高温影响植物的生长与繁殖，因而增强植物对高温的适应性是提高作物产量的重要途径。然而，对于植物是如何感知、转导高温胁迫的信号还有许多问题有待解决。本研究将分析水稻环核苷酸门控钙离子通道CNGCb和CNGCc参与调控高温反应的分子机制。项目前期已利用Crispr体系分别构建了CNGCb和CNGCc的突变体，并发现该突变体在高温处理下较野生型更为敏感，暗示其参与调控水稻高温反应。本项目将在此基础上，研究CNGCb和CNGCc参与调控高温反应的分子机制；分析其下游调控蛋白CaM和CBK是否参与调控水稻耐热反应；利用转录组分析探究CNGCb和CNGCc调控高温响应的转录机理和信号通路。通过的以上研究初步解析钙信号参与调控水稻高温胁迫反应的遗传通路和网络。本研究将提高对植物调控高温信号通路的认知，填补水稻CNGCs参与高温响应研究的空白，为进一步培育耐高温水稻品种提供一定的理论依据和思路。

High temperature is a limiting factor for the geographical distribution and the production of plants, and improving heat tolerance is critical for yield improvement of crop plants. It remains to be elucidated how temperature is sensed and signaled in plants to regulate various processes. The goal of this proposed research is to characterize the functions of CNGCb and CNGCc in regulating heat stress response in rice. We have used Crispr system to generate the homozygous cngcb and cngcc mutants, and identified that they were more susceptible to heat treatment, which indicates CNGCb and CNGCc may regulate thermosensory in rice. This study aims at further elucidating how calcium signal regulates heat tolerance through identifying the molecular basis and regulatory pathways of these two genes. In addition, we will also analyze the function of two potential CNGCs downstream mediator genes CaM and CBK in rice heat tolerance. These studied will enhance our mechanistic understanding of high temperature regulation in plants and fill the gap of functional analysis of rice CNGCs in heat stress, providing a theoretical basis for improving heat tolerance in crop plants.