通过再生医学的方法来构建具有促进成骨和抗炎作用的生物材料是治疗骨缺损的研究热点。骨形态发生蛋白（BMP-2）是最强的骨诱导剂，通过Smad信号通路促进成骨，但会被NF-кB通路抑制。微量元素锌（Zn）也具有成骨效应，其机制为拮抗NF-кB通路促进成骨。同时锌是良好的免疫调节剂，能抑制炎性因子的表达。因此，构建含锌和BMP-2的支架材料不仅能协同成骨且具有抗炎作用。本课题组在前期工作中已制备出一种新型的纳米无定形磷酸钙/聚乳酸（ACP/PLLA）复合材料，并利用该材料微观形貌自变化的特性构建了生长因子的缓释体系。本项目拟将锌和BMP-2整合入该支架材料中，构建BMP-2缓释型的ZnACP/PLLA复合体系。通过体外细胞实验评价该复合体系的成骨诱导和抗炎作用，并对材料中锌的抗炎机制及BMP-2和锌的协同成骨作用机制进行研究。将该复合材料体系植入骨缺损处，评价其在骨缺损中的修复效果和应用前景。

Based on the regenerative medicine approach, the fabrication of scaffolds with bone induction and anti-inflammatory ability to repair the bone defect has been a focus of attention. Bone morphogenetic protein (BMP-2) is the strongest growth factor for osteogenesis by activating Smad pathway. However, it will be suppressed by NF-кB pathway. Zinc, the trace element, also has its potential for osteogenesis by antagonazing NF-кB activation. Meanwhile, zinc is a good immunity modulator and can inhibit the production of inflammatory cytokines. Thus, the scaffold containing BMP-2 and zinc element has the effect on synergetic bone induction and anti-inflammation. In the previous work, we have developed a novel amorphous calcium phosphate/poly(L-lactic acid) (ACP/PLLA) material which could experience morphological variations in the microstructure and the growth factor controlled releasing system was then established. In this study, the BMP-2 controlled releasing and zinc containing ZnACP/PLLA composite is to be prepared. The composite is to be seeded with bone mesenchymal stem cells (bMSCs) and macrophages in vitro respectively. The effects on the osteogenesis and anti-inflammation together with their mechanisms are to be evaluated. The bone defect model is to be established, and BMP-2 controlled releasing ZnACP/PLLA composite is implanted and the efficacy for bone regeneration is evaluated.