皮肤区域特异性免疫调控分子SERPINB7对银屑病病理的作用及机制研究

银屑病是多基因遗传背景下的皮肤特异性自身免疫性疾病。本课题从银屑病样角质形成细胞出发，应用转录组测序，结合疾病基因组学、组织特异表达谱，筛选得到皮肤特异性银屑病免疫调控重要候选分子丝氨酸蛋白酶抑制因子SERPINB7。进一步结合临床相关性研究，系统地在Cre-LoxP条件性敲除动物模型及体外细胞模型中研究其对银屑病皮损免疫的调节作用。并通过对其结构与功能关系的精细解析以及皮肤特异性转录调控机制的深入研究，探讨皮肤特异性表达的丝氨酸蛋白酶抑制分子在皮肤免疫性疾病中可能具有的重要生理意义，为寻找更为特异的银屑病免疫治疗手段提供新思路。申请人有着多年自身免疫性疾病的研究经验，已经具有良好的研究基础，课题组良好的人员结构配置和有力的实验平台将会为课题的完成带来良好的支撑。

Psoriasis is a chronic inflammatory disorder of the skin with multiply genetic basis. In this study, we focused on the pathogenesis of psoriasis and highlight identifying molecules and genes in this disease.Based on psoriatic keratinocytes model, an integrative biology approach combined with transcriptome sequencing, disease genomics and tissue specific-expression profile enabled us to identify skin specific molecule serpinb7 that exerted a specific effect on psoriatic inflammation. Investigating the transcriptional regulatory mechanism and the relationship between the structure and functions have a very important significance on the study of psoriasis. These provide a new idea for finding more specific immune psoriasis therapeutic approach. Applicant has extensive experiences on the research of autoimmune diseases. The good personnel allocation and powerful experimental platform will bring us a good support for complete this study.