限进介质-分子印迹聚合物(RAM-MIPs)结合了分子印迹和限进材料的优点，能够同时实现对复杂样品中蛋白质的排阻和对目标小分子高选择性分离和富集的目的。目前的RAM-MIPs存在亲水性基团接枝密度低，印迹分子与识别位点结合效率低和洗脱困难的问题。本项目结合前期研究工作，采用表面引发原子转移自由基聚合（SI-ATRP）技术，在单分散交联聚甲基丙烯酸环氧丙酯树脂（孔径6-8 nm）表面接枝亲水性聚合物刷制备限进介质，选用农药或兽药为模板，再次采用SI-ATRP技术在该限进介质的表面制备RAM-MIPs固定相。以此固定相作为固相萃取柱，建立集样品前处理和分离检测为一体的在线联用方法，将其用于清真食品中痕量农兽药残留的高灵敏检测。本项目的实施，既解决了限进材料亲水性基团接枝密度低的问题，又提高了印迹分子与识别位点的结合效率，为RAM-MIP技术的发展提供了新方法。

Restricted access media-molecularly imprinted polymers (RAM-MIPs) combining the characteristics of RAM and MIPs, and realize the purpose of simultaneous protein removal and selective extraction and enrichment of small molecules in complex biological sample. However, the existing problems of RAM-MIPs are lower grafted density of hydrophilic groups, lower binding efficiency between the template molecular and the recognize sites and difficult to elute and re-bind the template molecular. Based on initial research of the project team, in this project, by adopting surface-initiated atom transfer radical polymerization (SI-ATRP) technique, the monodispersed poly(Glycidyl methacrylate-co-ethylenedimethacrlate) (PGMA/EDMA) particles as supporting matrix, prepare restricted access media by grafting hydrophilic polymer brush on the surface of PGMA/EDMA, then choose pesticide or veterinary drug molecules as templates, preparation of RAM-MIPs stationary phase re-using the SI-ATRP technology on the surface of the RAM. Using this stationary phase as SPE, the proposed method of pretreatment and separation detection the samples simultaneously is a basis of online SPE (RAM-MIP as sorbents) in combination with RAM-MIPs-LC-MS/MS, and applying to selectively detect trace pesticide and veterinary drug residues in Halal foods . Through the project implementation, not only slove the problem of lower grafted density of Hydrophilic groups, but also enhance the binding efficiency between the template molecular and the recognize sites, provides a new thought and method for the development of RAM-MIPs technology.