孕期弓形虫感染可垂直传播造成不良妊娠结局的发生。已证实蜕膜Treg细胞对于维持正常妊娠至关重要，申请人已研究发现，孕期弓形虫感染致不良妊娠结局的发生与孕鼠脾脏和胎盘Treg细胞数量及功能改变密切相关。本研究拟采用绿色荧光蛋白转基因（GFP-Foxp3 TG，Foxp3+Treg细胞呈自发绿色荧光）小鼠，将正常妊娠及早孕期弓形虫感染鼠Treg过继转输早孕期弓形虫感染鼠，观察对不良妊娠结局的影响，并采用活体成像系统观察外源性Treg被募集到蜕膜的数量及其与妊娠结局改善程度的关系，同时采用分子生物学方法检测Treg功能相关分子及治疗前后母胎界面各主要分子及细胞因子的表达水平。旨在探索过继转输Treg细胞对弓形虫感染致不良妊娠结局影响的分子机制。迄今未见相关报道。本研究组一直致力于弓形虫感染致不良妊娠结局发生机制的研究，本研究将为寻找减少弓形虫感染后不良妊娠结局发生的有效手段和方法奠定理论基础。

During human pregnancy, Toxoplasma gondii infection can cause unfavorable pregnancy outcomes through vertical transmission.It have been approved that decidual T regulatory(Treg) cells play an important role on sustain the normal pregnancy.In the previous study we have found that the change of the Treg's amount and functions contributed to abnormal outcome with Toxoplasma gondii infection during pregnancy.In this study the Treg cells were purifed from GFP-Foxp3 Transgenic mice, so the Treg cells were show an green flurescent because the recombinant GFP with Foxp3 protein expressed.The green flurescent Treg cells purifed from normal mice and Toxoplasma gondii infected mice respectively.The above two kinds of green fluresenct Treg cells will transfered into the Toxoplasma gondii infected BABL/c pregnant mice respectively to value their therapy effect on the abnormal pregnant outcome.The distribution and the amount of exotic Treg cells in the BABL/c mice were observed through vivi animal imaging system.The functional moleculares(CTLA-4,TGF- Foxp3) of Treg were analyzed with the methods fo moleculare biology after the therapy of transferred Treg cells. Also the relationship between the effect of transferred Treg cells and the abnormal pregnant outcome were analyzed.We expect this work may shed light on the molecular mechanism of the effect of transferred Treg on the abnormal pregnant outcome induced by Toxoplasma gondii infection during pregnancy.The exotic Treg cells will be provided a new therapeutic target for the treatment on adverse pregnancy outcomes of pregnant mice infected by T. gondii.The result of this study might be considered as potentially useful therapy to improve the abnormal pregnant outcome by Toxoplsma gondii infection during pregnancy.