Aux/IAA和ARF蛋白通过特异性互作在植物生长素信号转导和生长发育中起重要调节作用。目前相关研究主要集中于Aux/IAA和ARF蛋白特异性互作调控拟南芥、杨树等模式植物的正常生长发育，在木本植物嫁接研究领域少见报道。本项目拟在山核桃基因组和嫁接成活过程转录组测序的基础上克隆山核桃Aux/IAA和ARF基因家族成员，利用酵母双杂交、双分子荧光互补和免疫共沉淀技术鉴定山核桃Aux/IAA和ARF蛋白的特异性互作，利用原位杂交和免疫胶体金技术检测特异互作的Aux/IAA和ARF蛋白在山核桃嫁接成活过程中的表达量及定位，结合嫁接过程生长素含量测定，解析Aux/IAA和ARF蛋白特异性互作调控山核桃嫁接成活的分子机制，为揭示生长素调控木本植物嫁接成活的作用机制提供理论参考，为嫁接技术在生产中的广泛应用奠定实践基础。

The specific interactions between Aux/IAA and ARF proteins play important roles in auxin signaling pathway as well as growth and development of plants. Currently, related studies are focusing on the regulatory roles of specific interactions between Aux/IAA and ARF proteins in the normal growth and development in model plants like Arabidopsis and Populus, but the underlying mechanism of the specific interactions in tree grafting is little studied. In this project, based on the data from genome sequencing and transcriptome sequencing during the grafting process of Chinese hickory, members in the Aux/IAA and ARF gene family in Chinese hickory will be cloned; the specific interactions between members in Aux/IAA and ARF family will be identified by techniques of yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP); the expression levels and locations of specific interacting Aux/IAA and ARF proteins during the grafting process of Chinese hickory will be detected by in situ hybridization (ISH) and immune colloidal gold techniques; the dynamic changes of auxin during the grafting process will also be determined. This project aims to reveal the molecular mechanism mediated by the specific interactions between Aux/IAA and ARF proteins in successful grafting of Chinese hickory. Results from this project will not only provide theoretical references for understanding the mechanism of auxin in regulating the grafting process of trees, but also lay the foundation for the extensive application of grafting technique in production practice.