口腔黏膜下纤维性变癌变早期诊断分子标志物群的实验研究

目前，制约口腔癌及口腔黏膜癌前病变早期诊断的一个重要因素是特异性分子标志物的缺乏，而从体液与病变细胞质中筛选分子标志物又有方法学的问题和灵敏度的局限性。本研究项目提出建立口腔黏膜下纤维性变癌变分子早期预警与诊断的"分子标志物群"，实现口腔黏膜下纤维性变癌变早期诊断的新方法。本项目采用活版印生物芯片检测技术，高通量筛选口腔黏膜下纤维性变癌变特异性分子标志物；结合生物信息学分析方法，构建分子标志物多目标评价体系，寻找口腔黏膜下纤维性变癌变早期诊断"分子标志物群"， 用以揭示各分子标志物群在口腔黏膜下纤维性变癌变发生中所起的关键作用及其互相的作用机制。期待在分子标志物多目标评价体系的建立，"分子标志物群"的筛选、分子标志物互相的作用机理等方面取得原创性成果，为阐明口腔黏膜下纤维性变癌变关键机制提供新的信息，可望为口腔黏膜下纤维性变癌变的早期诊断开辟一条新的途径。

At present, an important factor of limited early diagnosis of oral cancer and oral precancerous lesions is a lack of special molecular markers, screening molecular markers from liquid and cytoplasm have still methodological and sensitive limitation. This researchful subjective structures molecular marker group of early diagnosis and forewarn for malignant transformation of oral submucous fibrosis and relize new method of early diagnosis for malignant transformation of oral submucous fibrosis. Special molecular markers of malignant transformation will be screenned by microarray and multiple target evaluable system will be structured by biological information technology for looking for molecular marker group of early diagnosis of malignant transformation of oral submucous fibrosis and discovering every molecular marker which plays an important role and active mechanism in malignant transformation of oral submucous fibrosis. An original innovation will be obtained in structuring multiple target evaluable system,screenning molecular marker group and active mechanism of molecular markers for obtaining new informations in key mechanism of malignant transformation of oral submucous fibrosis and new method of early diagnosis of malignant transformation of oral submucous fibrosis.