C型尿钠肽(CNP)提高了牛卵母细胞发育潜能，同时增加了线粒体DNA拷贝数，提示CNP可能调控了线粒体生物合成功能。PGC-1α是调节线粒体生物合成的核心因子，CNP是否通过激活PGC-1α调控牛卵母细胞线粒体生物合成，目前尚不清楚。本项目拟以CNP处理牛卵母细胞，通过检测PGC-1α基因表达、蛋白磷酸化和乙酰化，明确其是否激活PGC-1α；通过检测转录因子NRF1/2和TFAM、核基因组和线粒体基因组编码的线粒体蛋白基因表达以及线粒体数量和氧化磷酸化活性，明确激活PGC-1α是否促进了线粒体生物合成和氧化磷酸化代谢；通过调查是否激活CREB促进PGC-1α表达、激活AMPK使PGC-1α磷酸化或去乙酰化，解析激活PGC-1α的关键信号通路，探讨CNP调控牛卵母细胞线粒体生物合成的机制，为了解CNP提高牛卵母细胞发育潜能的原因提供有价值的信息，也为优化牛卵母细胞体外成熟体系提供理论依据。

C-type natriuretic peptide(CNP) improved bovine oocytes developmental potential, as well as increased mitochondrial DNA copy number, suggesting that CNP may regulate the function of mitochondrial biogenesis. PGC-1α is a transcriptional coactivator that plays a central role in this process of mitochondrial biogenesis. However, whether CNP regulates the function of mitochondrial biogenesis in bovine oocytes by activating PGC-1α remains unknown. In this study, bovine oocytes will be treated with CNP, then whether CNP activates PGC-1α will be determined by testing gene expression of PGC-1α, and its phosphorylation or acetylation; whether the increased activity of PGC-1α enhances mitochondrial biogenesis and oxidative phosphorylation metabolism in bovine oocytes will be clarified by examining the expression of NRF1/2, TFAM and mitochondrial protein genes encoded in nuclear and mitochondrial genomes, mitochondrial numbers and oxidative phosphorylation activity; The key signaling pathways involved in the activation of PGC-1α will be explored by investigating whether CNP enhances gene expression of PGC-1α, and its phosphorylation or deacetylation via activation of CREB and AMPK respectively. Based on the above results, we discuss the mechanism of CNP in control of mitochondrial biogenesis function in bovine oocytes. The implementation of this project would be able to provide valuable information for the understanding on improved developmental potential of bovine oocytes after treatment with CNP, and the theoretical basis for optimizing the culture system of bovine oocyte in vitro maturation.