肌细胞分化是由多种因子互作调控的动态过程，其中最重要的调控因子lncRNA功能解析尚处于新兴阶段且通用的研究方法是单向的（lncRNA到蛋白基因或反过来），与细胞内相互调控的机制存在差距。本研究利用肌细胞分化发育中具有全基因组调控能力并相互调控的核心因子H19(lncRNA)和MyoD（蛋白基因），以山羊骨骼肌卫星细胞为材料，整合转录组测序、CHIP-seq、FISH等技术，1）揭示山羊H19基因序列特点和表达规律；2）解析H19-MyoD轴调控骨骼肌分化的lncRNAs基因数目和特点，深度分析其中~5个核心lncRNAs基因；3）构建以H19-MyoD为轴，由lncRNAs、miRNAs以及编码基因组成的细胞分化调控网络。本研究将lncRNAs功能分析由目前的点线模式提升到网络模式；所构建H19-MyoD调控网络将能更准确反应细胞内基因间关系，是全面深入了解肌肉生长发育的重要基础。

Muscle cell differentiation is a dynamic process precisely controlled by the interactions among multiple factors. As the most important regulatory RNA, the functional research of lncRNAs is still in the emerging stage, and nowadays the popular study method focuses on one direction (from lncRNAs gene to protein gene, or vice versa), which result in a certain gap from reciprocal regulation characteristics between genes in reality. Taking advantage of the mutual regulationary interactions between MyoD (protein gene) and H19 (lncRNA gene), the core factors regulating gene expression genome-widely during muscle differentiation, and skeletal muscle satellite cells of meaty goat as material, and methods including RNA-seq, CHIP and FISH etc., we will investigate 1）characteristics and expression profiles of goat H19 gene; 2）characterize H19 – MyoD-axis related lncRNAs in regulating skeletal muscle differentiation, and analyze five to eight key lncRNAs well; and 3）construct an H19 - MyoD –axis core regulatory network of skeletal muscle differentiation which harbors several lncRNAs, miRNAs and encoding genes. This study will vastly elevate lncRNAs research level from the point-line model to the surface network mode in muscle differentiation. The H19 – MyoD-axis regulation network constructed will reflect the comprehensive relationships between genes in cell more precisely and found wonderful insights into the muscle growth regulatory mechanisms.