基于项目组前期研究结果，针对肿瘤分子成像与靶向治疗研究面临的关键技术问题（单模态及单功能分子探针诊疗效果有限，且难以穿透肿瘤组织屏障实现真正意义的分子成像与治疗），本项目拟研制一种新型多模态、多功能分子探针--肿瘤归巢穿膜肽介导的同时具有穿透双层肿瘤组织屏障、细胞外积聚和细胞内渗透作用的靶向载金属卟啉液气相变纳米粒（tLyP-1-MnPpIX-PFP NPs），利用tLyP-1的肿瘤靶向及穿膜特性引导纳米粒进入深层肿瘤组织细胞，实现细胞水平精准定位，联合LIFU使其相变成含气微泡，利用MnPpIX的磁导航及光、磁、声特性，实现超声分子成像、MRI分子成像、光学分子成像和光声成像；LIFU和UTMD导致的“爆炸效应”、MnPpIX声动力及光热治疗等多种疗法协同精准治疗肿瘤，实现“肿瘤细胞多模态分子成像”和“多效应协同全面杀伤肿瘤细胞”，为肿瘤多模态、多功能精准诊疗提供一种新策略和新方法。

Based on our previous research and focused on key technical problems faced by tumor molecular imaging and targeted therapy (unimodal and unifunctional molecular probes have limited theranostic effect, and it is difficult to penetrate the tumor tissue barrier to achieve “real” molecular imaging and targeted therapy inside tumor cells), this project will prepare a new multimodal and multifunctional molecular probe—targeted metalloporphyrin-loaded phase-transformation nanoparticles mediated by tumor homing-penetrating peptide with the characteristic of extracellular accumulation and intracellular penetration (tLyP-1-MnPpIX-PFP NPs). tlyp-1 peptide with the tumor targeting and penetrating characteristics can delivery nanoparticles into deep tumor tissue cells to realize the precise localization at cell level, and combined with phase-transformated microbubbles caused by low intensity focused ultrasound (LIFU), magnetic navigation and light, magnetic and acoustic characteristics of MnPpIX, which realize multimodal molecular imaging, such as ultrasound molecular imaging, MRI molecular imaging, optical molecular imaging and optoacoustic imaging. “explosion effect” caused by LIFU-UTMD, sonodynamic therapy and photothermal therapy of MnPpIX can achieve synergistic antitumor therapy. tLyP-1-MnPpIX-PFP NPs will realize “multimodal molecular imaging of tumor cells” and “multiple effects synergistically and comprehensively kill tumor cells”, which provide a new strategy and method for tumor multimodal and multifunctional precise theranostics.