侵袭性非霍奇金淋巴瘤（NHL）是最常见的一种淋巴瘤。尽管近年来其临床疗效有所改善，但侵袭性NHL仍是一种不可治愈的疾病，肿瘤缓解持续时间较短，很大一部分患者死于复发或难治。B7家族分子是调节T细胞免疫应答的重要分子，目前已发现其重要成员B7-H4在某些肿瘤发生发展起重要作用，但在侵袭性NHL中的研究尚未报道。本项目组前期研究发现侵袭性NHL细胞表达B7-H4，且通过炎症刺激后B7-H4分子表达明显上调。本课题拟以人侵袭性NHL肿瘤原代细胞及细胞系为研究对象，在已有的研究基础上，采用免疫学和分子生物学研究方法，通过研究侵袭性NHL肿瘤细胞异常表达B7-H4分子，观察T细胞增殖活化及CTL细胞抗肿瘤细胞能力的变化，进一步探索B7-H4介导侵袭性NHL细胞免疫逃逸中的具体作用及其可能机制，从而为T细胞为基础的免疫治疗提供新靶点。

Aggressive non-Hodgkin's lymphoma is the most common type of aggressive lymphoma. Although progress has been made in the management of aggressive NHL in the past decade, persistent remissions are usually not achieved and the management of patients with relapsed or refractory aggressive NHL is still challenging. The B7 family molecules play important roles in regulating T cell immune response. B7-H4, an important member of B7 family molecule,has been reported to play a vital role in the occurrence and development of certain tumors, which have not been reported in aggressive NHL. Our preliminary study proved that aggressive NHL cells expressed B7-H4, anf its expression upregulated after inflammation stimulation. On the basis of previous research, our project is intended to adopt human primary aggressive NHL tumor cell and aggressive NHL cell line as the research object, Use immunological and molecular biology research methods,study the abnormal expression of B7-H4 molecule, observe T cell proliferation and activation, clarify the specific immune escape mechanism of aggressive NHL cells mediated by B7-H4 molecule. The research results will provide new biological target for T cell-based immunotherapy.