间充质样运动和阿米巴样运动是细胞运动的两种基本形式。在肿瘤转移过程中，这两种运动形式相互转换促进了肿瘤转移。抑制肿瘤细胞运动形式的转换是抑制肿瘤转移的有效手段。然而，肿瘤细胞运动形式转换的调控机制仍不清楚。申请者前期的研究证实CD147可促进肝癌细胞的间充质样运动；AnnexinII可促肝癌细胞阿米巴样运动，那么CD147和AnnexinII能否调控肝癌细胞运动形式的转换从而促进肝癌转移，未见报道。本项目拟从CD147和AnnexinII对肝癌细胞运动形式的调控入手，应用CD147与AnnexinII基因敲除小鼠和基因敲除细胞等体内外模型，采用活细胞工作站、小动物活体成像、细胞3D培养等手段，原位、实时、动态监测肝癌转移过程中细胞运动形式的转换，研究CD147和AnnexinII调控肝癌细胞运动形式转换促进肝癌转移的的分子机制，全面认识肝癌转移过程，为肝癌的临床诊治提供新的线索和靶点。

Individual tumor cells exhibit two different modes of cell movement: mesenchymal mode and amoeboid mode. The two movement modes interconvert to promote tumor metastasis.Inhibiting interconversion of the two movement modes can be an effective way of inhibiting tumor metastasis. However, the regulation of their transition is unclear.Our previous study showed that CD147 promotes mesenchymal movement of hepatocellular carcinoma cells(HCC); AnnexinII promotes amoeboid movement of HCC.But it has been not reported whether CD147 and AnnexinII may regulate the transition of mesenchymal mode and amoeboid mode.In this study,we use gene knockout mice、gene knockout cell lines、live cell workstation、small animal imaging、the 3D cell culture system to explore the role of CD147 and AnnexinII in the regulation of cell movement modes transition and clarify the mechanism of HCC metastasis.Thus giving rise to the promise of new clue to inhibit metastasis in HCC treatments.