杉木是我国重要的用材树种，其造林面积和材积量均位列主要造林树种首位。杉木炭疽病是我国杉木产业健康发展的主要威胁之一，但目前该病的致病机制还不是很清楚。去磷酸化作用是蛋白功能调控的重要方式，丝氨酸/苏氨酸(Ser/Thr)磷酸酶介导的去磷酸化作用对真核生物的生物学过程具有重要调节作用。PPZ1是真菌特有的一类Ser/Thr磷酸酶，该基因在植物病原真菌中的功能及其调控机制目前还不清楚。项目前期从杉木炭疽菌中鉴定到一个PPZ1蛋白同源物CgPPZ1，发现该基因的缺失突变体致病力显著降低。本项目将在此基础上，全面解析CgPPZ1在杉木炭疽菌生长、胁迫抗性及致病等方面的功能；鉴定与其互作的调控蛋白2-3个，并解析调控蛋白与CgPPZ1的互作机制，从而系统揭示CgPPZ1的生物学功能及其调控机制。研究结果将有助于揭示杉木炭疽病菌的致病分子机制，并为筛选新型的杀菌剂作用靶标提供重要理论基础。

Chinese fir (Cunninghamia lanceolata) is an important fast-growing trees species in China. Both of its afforestation area and timber volume are ranked in the first place among afforestation trees species. Anthracnose is widespread in the plantation area of Chinese fir and becomes one of the major threats to the Chinese fir industry. However, the pathogenic mechanism of this disease is still not clear. Dephosphorylation is an important way to regulate the function of target proteins, and serine/threonine (Ser/Thr) mediated dephosphorylation has a crucial role in regulating the biological processes of eukaryotes. PPZ1 is one type of Ser/Thr phosphatases unique to fungi, but the function of this enzyme and its regulatory mechanism in phytopathogenic fungi are still unclear. A PPZ1 homologue, CgPPZ1, was identified from Colletotrichum gloeosporioides. It was found that this gene is involved in appressorium development and virulence of C. gloeosporioides. This project will analyze the potential biological functions of CgPPZ1 in growth, stress resistance and pathogenicity, and identify 2 to 3 regulatory proteins, and analyze the interaction mechanism between regulatory proteins and CgPPZ1, and systematically reveal the function and regulation of CgPpz1. These results will help to further reveal the pathogenic mechanism of C. gloeosporioides on Chinese fir, and provide an important theoretical basis for screening of novel fungicides. At the same time, it will supply important reference value for analyzing pathogenic mechanism of other woody pathogenic fungi.