微生物感染无时无刻不在威胁着人类的健康，随着越来越多耐药性菌株的不断出现，微生物对传统抗生素的耐药性问题已经成为一个严峻问题亟待解决。本项目立足于这一迫切需求，致力于设计制备新型的高效低毒广谱抗菌化合物。细菌的细胞壁中含有大量的多糖类物质，申请人在前期预研中发现多糖基阳离子抗菌材料具有良好的广谱抗菌性能和较低的动物细胞毒性，并基于实验结果进一步提出了多糖基抗菌材料因其与微生物细胞壁组分的结构具有相似性而对微生物具有较高的选择性这一观点。基于此观点，本项目拟利用“点击”化学的策略，通过构建多种可点击的多糖和多肽片段，便捷地合成出多种结构的“肽多糖”类抗菌高分子化合物，并系统地评价所合成材料的抗菌性能和动物细胞毒性等生物学性能，力图从中筛选出新型高效低毒的抗菌高分子材料，同时进一步探索材料结构与抗菌性能之间的关系。

Microbial infections endanger public health unremittingly and give rise to a huge economic burden to our society. With the rapid rise of antibiotic-resistant strains, microbe antibiotic resistance is now considered to be one of the most imperative public heath issue that needs a new solution. Hence numerous efforts have been made to develop new antimicrobial molecules which are able to inhibit pathogens. In this project, we are intend to develop novel broad-spectrum antimicrobial molecules which are high effective against microbes and low toxic to human cells. In our recent research, we have designed novel polysaccharide based membrane-active antimicrobial molecules, which show excellent broad-spectrum antimicrobial activity and low cytotoxicity. We postulate that antimicrobial molecules designed to resemble bacterial cell wall constituents may be less hemolytic than present AMPs and AMP analogues due to their dissimilarity to mammalian outer membrane constituents. Base on this theory, we propose several polysaccharide-based structures for antimicrobial application, using "click" chemistry. The mechanisms of the novel polysaccharide-based antimicrobial polymers will also be investigated.