蛹虫草（Cordyceps militaris）作为传统中药及新资源食品，在我国广泛栽培，年产量达到1000吨干重。但是菌种退化带来的子实体产量及品质下降、甚至完全丧失子实体形成能力，造成巨大的经济损失。研究表明，脂类代谢与真菌退化密切相关，Nem1-Spo7蛋白复合体对脂类的合成、调控有重要作用。对蛹虫草中Nem1-Spo7前期研究发现，该复合体调控细胞内脂类的合成及子实体的形成，过量表达CmNem1基因能有效恢复菌株产生子实体的能力。本项目拟进一步对蛹虫草野生型菌株和突变菌株的生理生化、细胞结构、形态发育、子实体形成等多个层面开展比较分析，探求蛹虫草中Nem1-Spo7复合体参与的生物学过程，结合基因表达与蛋白定位、蛋白质磷酸组学分析、脂代谢谱分析等，明确Nem1-Spo7在子实体形成中的作用机理，为探寻真菌子实体形成及退化机理奠定良好的基础。

As a traditional Chinese medicine and a new resource food, Cordyceps militaris is widely cultivated in China with an annual output of 1000 tons. However, degeneration of fungal varieties reduced the yield and quality of the fruiting body, and even led to complete loss in the fruiting body formation ability, which caused huge economic loss. Studies have shown that lipid metabolism is closely related to fungal degeneration, and the Nem1-Spo7 protein complex plays an important role in the synthesis and regulation of lipids. The previous studies on Nem1-Spo7 of Cordyceps militaris showed that, this complex regulates the synthesis of lipids in cells and the formation of the fruiting body, and the overexpression of CmNem1 gene can effectively restore the ability of the production of the fruiting body. This project intends to further study the morphology, physiology, biochemistry, cellular structure of fruiting body formation of the wild type strain and mutant strains, etc. Comparative analysis will be carried out to explore the CmNem1-Spo7 complex involved in biological processes in cordyceps militaris. Analysis of gene expression, protein localization and lipid metabolism spectrum will be combined to figure out the role of CmNem1-Spo7 in fruiting body formation and degeneration mechanism. The project will lay a good foundation for the exploration of the fungus fruiting body formation and degeneration mechanism.