阿司匹林作为百年老药，它能影响动物甚至是植物体内多种生理活动，被广泛应用于临床各领域。多项临床流行病学研究数据表明，阿司匹林可预防癌症的发生及降低癌症的死亡率。阿司匹林抑制肿瘤具体机制尚未完全阐明。肿瘤耐药是目前导致肿瘤治愈率不高及转移的主要原因，联合用药是目前临床抗肿瘤治疗主要方法。本项目根据现有的研究成果及待解决的科学问题，决定以阿司匹林抗肿瘤作用为突破口，阐述阿司匹林抗乳腺肿瘤作用及提高抗癌药物他莫昔芬、顺铂敏感性的分子机制。同时，建立耐他莫昔芬、顺铂乳腺肿瘤细胞系，利用肿瘤分子生物学和细胞生物学等科研手段，筛选亲本细胞与耐药细胞差异基因，探究差异基因与肿瘤细胞侵袭、转移、增值和成瘤能力之间的相互关系，阐明差异基因作为抗肿瘤的生物学靶点的意义并结合肿瘤微环境，从根本上阐述阿司匹林在乳腺肿瘤发生发展过程中的关联作用，为揭示乳腺肿瘤的发生及转移机理和为乳腺肿瘤有效的防控提供理论依据。

As a hundred years old drug, aspirin has an extremely magical property. It can affect various physiological activities of animals and even plants, it is widely used in every clinical fields. A flurry of clinical epidemiological data showed that aspirin protected from cancers and reduced the rate of mortality . The exact mechanism by which aspirin inhibits tumors has not been fully elucidated. At present, tumor drug resistance is the leading cause of tumor low cure rate and the main reason for the tumor metastasis, drug combination is the main method of clinical anti-tumor therapy. Based on the existing research results and the unsolved scientific issues, this project decided to take the antitumor effect of aspirin as a breakthrough to expound the negative effect of aspirin to breast neoplasms and the molecular mechanism of enhanced sensitivity to tamoxifen and cisplatin. At the same time, we establish drug resistance cell lines to tamoxifen and cisplatin, we also use the oncomolecularbiology, cytobiology and other scientific research methods to screen differentially expressed genes between parental cells and drug-resistant cell, to explore the correlate between differentially expressed gene and the ability of invasion, metastasis, proliferation and formation of tumor cells, clarify the significance of differential expressed gene as a biological target of anti-tumor and integrate the tumor microenvironment, the project fundamentally represented related role which aspirin played in the development and progression of mammary gland tumors in order to reveal the occurrence and metastasis mechanism of mammary gland tumors and provide theoretical foundation for the prevention and control measures.