Combined detection of CA15-3, CEA, and SF in serum and tissue of canine mammary gland tumor patients.
Combined detection of CA15-3, CEA, and SF in serum and tissue of canine mammary gland tumor patients.
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犬乳腺肿瘤患者血清和组织中CA15-3、CEA、SF联合检测
DOI：
10.1038/s41598-021-85029-4
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发表时间：
2021-03-23
影响因子：
4.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Fan Y;Ren X;Liu X;Shi D;Xu E;Wang S;Liu Y
本研究旨在探讨CA 15 -3、CEA和SF在犬乳腺肿瘤中的表达水平及其临床诊断价值。本研究采用ELISA和qRT-PCR方法检测了178例CMGT患者和健康犬的组织/血清CA 15 -3、CEA和SF水平。恶性肿瘤组CA 15 -3、CEA、SF水平明显高于良性肿瘤组和健康对照组。在恶性肿瘤组中，CA 15 -3的敏感性为51.8%，特异性为93.9%，准确性为76.8%。CEA诊断胃癌的敏感性、特异性和准确性分别为44.6%、84.1%和68.1%。SF的敏感性为62.5%，特异性为85.4%，准确性为76.1%。SF的敏感性最高，CA 15 -3的特异性最高。三种生物标志物联合检测在恶性肿瘤组中的敏感性、特异性和准确性分别为80.4%、78.0%和80.0%，因此联合检测可提高敏感性和准确性，但降低特异性。结论：血清/组织标志物CA 15 -3、CEA、SF联合检测可提高CMGT的检测灵敏度，为临床应用提供参考价值。
The purpose of this study is to evaluate the levels and clinical diagnosis value of CA15-3, CEA, and SF in canine mammary gland tumors (CMGTs). In this study, the levels of tissues/serum CA15-3, CEA, and SF in 178 CMGT patients or healthy dogs were determined by ELISA and qRT-PCR assay. CA15-3, CEA, and SF levels of the malignant tumor group were significantly higher than that of the benign tumor group and the healthy control group. In the malignant tumor group, CA15-3 held a sensitivity of 51.8%, a specificity of 93.9%, and an accuracy of 76.8%. The sensitivity, specificity, and accuracy of CEA were 44.6%, 84.1%, and 68.1% respectively. SF held a sensitivity of 62.5%, a specificity of 85.4%, and an accuracy of 76.1%. SF showed the highest sensitivity and CA15-3 showed the highest specificity. The sensitivity, specificity, and accuracy of the combined detection of the three biomarkers in malignant tumor groups were 80.4%, 78.0%, and 80.0%, respectively, therefore combined detection increased sensitivity and accuracy but decreased specificity. In conclusion, the combined detection of serum/tissue markers CA15-3, CEA, and SF may improve the detection sensitivity of CMGTs, providing reference value for clinical application.
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