洞庭湖湿地的重金属污染对水生生物乃至人类的健康构成了严重威胁。微生物修复技术因其处理成本低和对环境友好等优点已经成为重金属污染土壤或底泥修复的研究热点，但应用过程中其性能受到修复效率和体系稳定性的制约。将微生物与氯磷灰石联用是高效修复重金属污染的一种有效途径。本项目以东洞庭湖湿地底泥为研究对象，拟构建白腐真菌耦合氯磷灰石联合修复体系，明确环境因子的影响规律，对联合修复体系的修复性能进行优化调控；研究白腐真菌与氯磷灰石的耦合强化效应及交互作用机理，并利用现代表征技术对修复产物进行分析，揭示白腐真菌耦合氯磷灰石强化修复底泥重金属污染的行为机制；最后研究联合修复体系中底泥理化性质、微生物酶活性和底泥微生物群落结构的变化，综合评价联合修复技术应用于重金属污染底泥修复的生态安全性。本研究有望为开发廉价、高效、稳定和安全的重金属污染湖泊湿地修复技术提供理论依据与技术支持。

The heavy metal pollution in Dongting Lake Wetland poses a serious threat to the health of aquatic organisms and even humans. Microbial remediation technique has become a hot research for the remediation of heavy metal contaminated soil or sediment because of its low processing cost and environmental friendliness. However, the performance of microbial remediation technique is restricted by the remediation efficiency and system stability. The combination of microorganisms and chloroapatite is an effective way to efficiently repair heavy metal pollution. Therefore, this project takes the sediment of East Dongting Lake Wetland as the research object, and a combined remediation system of white rot fungus coupled with chloroapatite is constructed, the influence law of environmental factors is clarified and the performance of the combined remediation system is optimized. The coupling strengthening effect and interaction mechanism of white rot fungus and chloroapatite are studied, and the remedied products are analyzed by the modern characterization techniques, and the remediation mechanism of white rot fungus coupled with chloroapatite in strengthening the remediation of heavy metal contaminated sediment is revealed. Finally, the changes of the physical and chemical properties of sediment, the microbial enzyme activities and the microbial community structure are observed, and the ecological safety of using the combined remediation technique to repair heavy metal contaminated sediment is comprehensively evaluated. This study is expected to provide theoretical basis and technical support for the development of low-cost, efficient, stable and safe techniques for remediation of heavy metal contaminated lake wetland.