类黄酮是植物体内重要的次生代谢物质之一，在植物生长发育中发挥重要的作用。银杏是重要的药用经济林木，银杏叶片高积累黄酮类化合物，但其背后的遗传机制尚不清楚。基于前期研究基础，本项研究以10个不同野生、半野生群体共298株古银杏中筛选出高积累类黄酮和低积累类黄酮的银杏单株为材料，通过代谢组学、转录组学、基因组学及生物信息学，多组学交叉融合的方法筛选参与银杏类黄酮代谢过程中的关键基因，以及相关MYB转录因子，并通过遗传转化银杏愈伤组织探索关键基因的功能。同时，基于酵母双杂、双分子荧光互补（BiFC）和Pull down技术筛选与关键基因互作蛋白；基于酵母单杂、凝胶阻滞（EMSA）和CHiP-seq测序技术筛选与关键基因互作转录因子。解析银杏叶片类黄酮代谢机制，丰富植物类黄酮代谢调控理论，进而为银杏叶用林良种选育建立理论基础。

Flavonoid was one of the most important secondary metabolites in plant and played an important role in plant growth and development. Ginkgo (Ginkgo biloba L.) was an important economic tree with medicinal value for its high contents of flavonoids in leaves, but we have not been able to explain the genetic mechanism clearly up to now. Based on our previous research, we successfully selected high and low accumulation of flavonoids clones in 298 ancient wild and semi-wild ginkgo trees from 10 geographical populations as our research materials. We wanted to screen and identify the key genes and related MYB transcription factors that participated in the flavonoid metabolism through multiple methods, such as transcriptomics, metabolomics, genomics and bioinformatics, and deeply explore the possible biological function with transgenic ginkgo callus. Meanwhile, we used the Yeast Two-Hybrid System, and Bimolecular Fluorescence Complementation, Pull down methods to screen and identify the possible interacting protein of the key genes. We also used the Yeast One-Hybrid system, Electro Mobility Shift Assay and CHiP-seq to screen and identify the possible interacting transcription factors of the key genes. We hope to illuminate the gene regulation network and clarify the genetic mechanism of flavonoid metabolism in Ginkgo. The research results were effective supplements for flavonoid metabolism theory in plant and lay a solid foundation for future leaf-harvest gingko genetic breeding and cultivation.