猪场废水厌氧消化液具有高氮低碳特性，脱氮处理困难，传统硝化-反硝化工艺已无法满足其脱氮需求。自养型同步脱氮(ANR)颗粒污泥，绿色高效、经济环保，是目前最具前景的废水脱氮技术之一。本课题组的前期研究发现原本厌氧氨氧化细菌在内、好氧氨氧化细菌在外的ANR颗粒污泥，在处理猪场废水厌氧消化液后，形态逐渐分型，形成以厌氧氨氧化细菌为主的颗粒污泥部分和以其它细菌为主的絮体污泥部分，但是导致该现象的分子机制尚不明确。本项目拟围绕ANR颗粒污泥形态分型的现象，明确猪场废水厌氧消化液中导致形态分型的主效因子；利用宏基因组、宏转录组、生物化学和分子生物学等研究手段，解析厌氧氨氧化细菌中与胞外蛋白及多糖合成相关的生物膜形成通路，并与絮体污泥优势菌群的生物膜形成通路进行比较，从而揭示ANR颗粒污泥形态分型的分子机制，以期为ANR颗粒污泥处理猪场废水厌氧消化液的效能提升奠定理论基础。

Anaerobically digested swine wastewater contains high amounts of ammonium, but its organic carbon is not sufficient to satisfy the demand of the traditional nitrification-denitrification process. Autotrophic nitrogen removal (ANR) granular sludge is a cost-effective and environmentally friendly technology, which would be the most promising notrogen removal technology from wastewater. In previous research we found that the ANR granular sludge mutated from an integrated granules with the interior anaerobic ammonia oxidizing (ANAMMOX) bacteria and the exterior aerobic ammonia oxidizing bacteria (AOB) into two parts including granules parts with ANAMMOX bacteria and flocs parts with AOB and other bacteria, when the influent gradully changed from synthetic wastewater to digested swine wastewater. It suggested that there are some granular sludge disassembly factors in digested swine wastewater, but ANAMMOX bacteria have some molecular mechanism to tolerate these factors. In this project, it is proposed to use metagenome, metatranscriptome, chemistry and molecular biology methods to identify the main factors causing morphological classification of ANR granular sludge in digested swine wastewater; analyze the genes of extracellular proteins and exopolysaccarides of ANAMMOX bacteria; reveal the molecular mechanism of sludge morphological classification by comparing the biofilm formation pathways of ANAMMOX bacteria and dominant bacteria in floc part. It will lay a key foundation for enhancing the performance of ANR granular sludge to digested swine wastewater.