保幼激素(JH)对家蚕的生长发育和蚕丝蛋白合成至关重要。然而，关于家蚕保幼激素的生物合成调控，我们还知之甚少。我们发现干涉POU-M2后，家蚕提前蜕皮和变态化蛹。免疫组化分析发现POU-M2在家蚕的咽侧体中表达。RT-PCR分析表明POU-M2在咽侧体中的表达谱与JH滴度的变化趋势一致。过表达POU-M2后，JH合成信号通路中的关键酶和Kr-h1都上调表达；干涉POU-M2后，JH合成信号通路中的关键酶和 Kr-h1全部下调表达。双荧光素酶分析表明POU-M2可以促进保幼激素甲基转移酶(JHAMT)启动子的转录。因此，我们推测POU-M2在家蚕咽侧体中调控了JHAMT的转录，从而调控了JH的生物合成和家蚕的生长发育。研究POU-M2调控家蚕保幼激素生物合成的分子机制，不仅有助于更好地理解家蚕和鳞翅目昆虫生长发育的调控机制，还可以为农林业害虫的生物防控提供新的分子靶标和理论指导。

Juvenile hormone (JH) is crucial for the growth and silk protein synthesis of the silkworm, Bombyx mori. However, little is known about the regulation of juvenile hormones biosynthesis in the silkworm. We found that the molting and pupation of silkworm occurred in advance after the interference of POU-M2. Immuno-histochemical analysis revealed that POU-M2 was expressed in the corpora allata (CA) of the silkworm. RT-PCR analysis showed that the expression profiles of POU-M2 in the CA of silkworm were consistent with the trend of JH titer in the hemocyte. The expressions of the key enzymes in the JH biosynthesis signal pathway and Kr-h1 were up-regulated after the overexpression of POU-M2, and down-regulated after the interference of POU-M2. Dual luciferase assays showed that POU-M2 promoted the transcription of juvenile hormone acid methyltransferase (JHAMT) promoter. Therefore, we suggested that POU-M2 regulates the transcription of JHAMT in the corpora allata of the silkworm, thus regulating juvenile hormones biosynthesis and the development of silkworm. Studying the molecular mechanism of juvenile hormone biosynthesis regulated by POU-M2 in the silkworm not only facilitates to better understand the regulatory mechanism of the silkworm and lepidopteran insects development, but also could provide new molecular targets and theoretical guidance for the biological control of agricultural and forestry pests.