遗传重组对生物进化和物种形成至关重要，同时也是农作物利用遗传多样性进行新品种培育的基础。然而，遗传重组较低的发生频率及非随机分布等规律所导致的遗传累赘，限制了新品种培育过程中优良基因的聚合。因此，系统解析遗传重组发生和分布规律对遗传育种具有重要的意义。本项目拟选取籼粳杂交稻品种春优84为模式材料，利用CRISPR/Cas9基因编辑系统编辑ZEP1基因的外显子以及HEI10基因的启动子和uORFs区域，创制遗传重组频率提高的材料，基于多重PCR及搭桥序列拼接技术，开发适合重组后代大样本多位点的二代测序方法，编写以高密度杂合SNPs为标记的重组自动分析软件，对野生型和编辑后不同突变体及突变组合材料的重组频率和分布规律进行系统分析，解析杂交水稻遗传重组的发生规律，以及不同重组相关基因突变对遗传重组发生和分布的影响，为利用基因工程手段实现对重组频率和位置的调控、打破遗传累赘、提高育种效率奠定基础。

Genetic recombination is not only essential for biological evolution and speciation, but also a major source of genetic diversity in crop breeding. However, the linkage drag caused by the limited number and non-random distribution of genetic recombination severally hinders the aggregation of excellent genes during plant breeding. Therefore, systematic analysis of the occurrence and distribution of genetic recombination is of great significance for genetic breeding. In this project, the hybrid rice variety Chunyou 84 is selected as the model variety. The exon of ZEP1 gene and the promoter and uORFs of HEI10 gene will be respectively edited using CRISPR/Cas9 genome editing system to create plants with improved genetic recombination frequency. Also, we plan to develop a next generation sequencing method suitable for large-scale analysis of the recombinant progeny based on multiplex PCR and bridging sequence splicing technology. In addition, automatic analysis software based on high-density heterozygous SNPs will be compiled. Finally, we will systematically investigate the recombination frequency and distribution of the wild-type hybrid and the mutants, which will promote the understanding of the roles of the two genes during recombination. The work will lay a foundation for the breeders to gain control of genetic recombination, break the linkage drag, and improve the breeding efficiency in the future.