除虫菊为菊科观赏植物，以产生安全无毒、环境友好的天然杀虫剂除虫菊酯而著称，其天然产品供不应求。目前其除虫菊酯代谢路径的机制尚不清楚。菊基二磷酸合成酶（CDS）是新近发现催化其前体二甲烯丙基二磷酸（DMAPP）产生菊基二磷酸（CPP）的关键基因，有趣的是我们以除虫菊CDS基因转化高感蚜虫的菊花时腺体中却检测到CPP的下游产物菊基醇（COH）；而且，前人认为除虫菊酯含量与腺体密度相关，但我们检测腺体中却没有除虫菊酯。本项目拟在前期除虫菊（与菊花）腺体观测、遗传转化体系建立基础上，通过不同器官和发育阶段腺体与分泌管观测、内含物测定及其除虫菊酯细胞定位、CDS基因和启动子转化除虫菊、蛋白体外表达和细胞溶解反应等，探究腺体与CDS产物表达以及除虫菊酯合成、贮运的空间关联，解析CDS功能及其双重调控除虫菊酯前体的机理。本研究对揭示整个除虫菊酯代谢机制、提高除虫菊酯含量和菊花抗虫性具有重要的理论意义。

Pyrethrum (Chrysanthemum cinerariifolium,syn. Tanacetum cinerariifolium) is ornamental plant of Composite family,and is well-known as a safe and non-toxic natural pesticide of pyrethrins, the supply has not been adequate to the demand.However, the internal mechanism of pyrethrin biosynthasis pathway is unclear to date. Recently, chrysanthemyl diphosphate synthase (CDS) was reported to be the first key synthase to catalyse the precusor dimethylallyl diphosphate (DMAPP) into chrysanthemyl diphosphate (CPP). Interestedly, we found that the transgenic CDS chrysanthemum plant produced chrysanthmol (COH)and expressed in the leaves glandular tricomes;Furthermore,the pyrethrin content,which was reprorted to be related to tricomes,has not been detected in pyrethrum tricomes in our experiments.Thus, this study is conducted to determine the CDS gene function by observing the glandular tricomes,secretion structures and cellular localization of pyrethrin in transgenic pyrethrum plants,and cell lysate reaction is proposed to investigate the bifunctional enzymes of CDS and the relationship with pyrethrin biosynthesis, storage and transportation. The study would provide informative theoretical basis and practical foundation for molecular modified breeding in pyrethrum and chrysanthemum by revealing the pyrethrin metabolism.