植物次生代谢产物是中药安全有效的物质基础。内生真菌种群分布与中药活性成分相关，单菌回接植物或刺激愈伤组织、毛状根等均影响活性成分形成和积累。然而植物与多种内生真菌共栖的秘密有待阐明。基于“地造灵药”是土壤重构植物微生态后产生的生理生态适应性变化的假说，初步发现不同种源丹参移栽后形态特征稳定，再生苗不同于原产地和根段栽培的是活性成分含量趋于接近。推测土壤重组了再生苗的微生态系统，引起光合生理和根际效应适应性变化，改变了植物微生态系统内部和内外物质流，形成的相似体内环境。本研究以丹参为载体，利用光合作用仪、HPLC-MS、ICP-MS、DGGE和第二代测序技术等，分析重构“植物+微生物”在无菌、温室和大田生境下，真菌组成—光合生理—根际效应—药材品质之间的互作关系。以期揭示内生真菌影响丹参质量和产量的生理生态机制，建立丹参品质定向调控的新方法和新技术，为药用植物品质定向调控提供新思路和新方法。

Pant secondary metabolites is material base of pharmacological characteristics. The structure of populations of endophytic fungi population structure associated with effective composition of herbes. Influence on the formation and accumulation of effective composition, such as single strains inoculated plants or on callus and hairy root, etc. Yet, because of fungal endophytes sheer number and diversity,modes of action still await rigorous demonstration for most host plant symbionts.Recently, we hypothesized that soil reconstructe the plant micro-ecology, change eco-physiological adaptability basing on TCM philosophy of "Good medicine from land".Our preliminary study indicated that contents of effective composition of Salvia miltiorrhiza by cutting root reproduction maintain differences of origin which come from sichuan, shandong, henan province. Morphological characteristics of seedling regeneration of Salvia miltiorrhiza is stability, but contents of effective composition is close to each other.So,we think the micro ecosystem of the regenerated seedlings restructuring in soil, the photosynthetic physiology and rhizosphere behavior to adapt to change, change the system of internal and external material flow, formed the approximate internal environment. This research can be used Salvia miltiorrhiza as model medicinal plant for studying endophyte -photosynthetic physiology –rhizosphere effects-effective composition four interact with each other,when regeneration seedling culture in sterile, greenhouse and field environment,with HPLC-MS, icp-ms, DGGE and the second generation sequencing technology. Thus, demonstrating that eco-physiological mechanisms of the change of the quality of Salvia miltiorrhiza by endophytic fungi infection, establishing a new methods and techniques of directional control quality of salvia miltiorrhiza. also provide new ideas and new methods of directional control quality of medicinal plants.