本项目针对水牛胚胎干细胞在培养过程中容易丧失自我更新能力，无法长期稳定传代的问题，以细胞葡萄糖代谢途径的转换与自我更新能力的关系为切入点，拟采用细胞能量代谢表型测定、RNA-seq、ChIP-qPCR和RNA干扰/过表达等技术手段，系统探讨氧化磷酸化和糖酵解这两条葡萄糖代谢途径在维持水牛胚胎干细胞自我更新能力的作用；重点探索Wnt/β-catenin-HIF1α信号通路在维持水牛胚胎干细胞自我更新中的作用及调控葡萄糖代谢途径转换的应答机制。本研究将为水牛和其他大家畜胚胎干细胞培养系统的改良提供新的理论依据，并有望获得能长期稳定传代的水牛胚胎干细胞系，为加速广西本地水牛品种的改良，加快我国南方水牛奶业的发展奠定基础。

This project aims to solve the problems that buffalo embryonic stem cells can’t be stably passaged and lose their ability of self-renewal during culture. In order to explore the role and its response mechanism of glucose metabolic pathways switch in the maintenance of buffalo embryonic stem cells self-renewal mechanism, this project is undertaken to systematically investigate the role of oxidative phosphorylation and glycolysis in the maintenance of buffalo embryonic stem cell self-renewal ability by using cell energy metabolism detector, RNA-seq, ChIP-qPCR and RNA interference/overexpression techniques; focus on exploring the role and response mechanism of Wnt/β-catenin-HIF1α signaling pathway in the switch of glucose metabolism and maintaining self-renewal of buffalo embryonic stem cells. These research works will provide new insights for the improvement of buffalo and other large livestock embryonic stem cell culture conditions, and prospectively establish the true buffalo embryonic stem cell lines.