调节性T细胞（Tregs）稳定AS斑块研究正受到广泛关注。最近研究证实，激活PPARγ可显著促进Tregs聚集。前期研究发现解毒活血抗炎经典名方四妙勇安汤可激活PPARγ稳定ApoE-/-小鼠AS斑块，体外研究显示其核心药用部位CS05具有激活PPARγ作用。由此，我们提出“CS05激活PPARγ募集Tregs ，抑制炎症，稳定AS斑块”假说。拟基于ApoE-/-小鼠AS斑块模型和3T3-L1前脂肪细胞分化模型、ox-LDL诱导原代脾细胞、THP-1与Tregs共培养细胞实验，明确其活性部位CS05是否具有激活PPARγ，募集Tregs的药理作用，进而以罗格列酮和PPARγ阻断剂为对照，从体内外探讨CS05激活PPARγ促进Tregs累积，抑制炎症反应，稳定AS斑块的分子机制。本课题有望开拓中医药防治AS药理作用及机制研究的新思路，为研制具有募集Tregs的天然PPARγ激动剂奠定基础。

Regulatory T cell Tregs stabilizating atherosclerotic plaques have been paid more and more attention.Recent studies have shown that activation of PPAR γ can significantly promote Tregs aggregation.Previous studies showed that Si-miao-yong-an decoction could activate PPAR γ to stabilize ApoE-/- AS plaque,and studies in vitro showed that its core medicinal site CS05 could activate PPAR γ significantly.Therefore, we propose the hypothesis that CS05 activates PPAR γ recruitmentTregs, inhibits inflammation and stabilizes AS plaques.In this experiment,we adopt the ApoE-/- mouse as plaque model and the 3T3-L1 adipocyte differentiation model , through experiment of primary spleen cells induced by ox-LDL, co culture experiment of THP-1 and Tregs ,to clarify its pharmacological effects as ligands to activate of ppar γ and recruit of tregs ,then rosiglitazone and PPAR γ blockers are used as control to study the molecule mechanism of CS05 on activating PPAR γ to promote Tregs accumulation, to inhibit inflammatory response and stabilize AS plaque .In this paper, a new idea on the pharmacological action and mechanism of traditional Chinese medicine (TCM) in the prevention and treatment of AS will be developed, which lay a foundation for the development of natural PPAR γ agonists with Tregs recruitment.